Mouse IL-12 R beta 1 Biotinylated Antibody Summary
Val32-Glu561
Accession # Q60837
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12 R beta 1
IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G‑CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16%‑21% aa sequence identity with the ECDs of mouse gp130, LIF R, G‑CSF R, and IL‑23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL‑23 R (4‑6). IL‑12 R beta 2 and IL‑23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8‑10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).
- Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
- Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
- Chua, A.O. et al. (1995) J. Immunol. 155:4286.
- Parham, C. et al. (2002) J. Immunol. 168:5699.
- Wu, C. et al. (1997) J. Immunol. 159:1658.
- Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
- Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
- Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
- Airoldi, I. et al. (2000) J. Immunol. 165:6880.
- Li, J. et al. (2003) J. Neurol. Sci. 215:95.
- Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
- Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
- Shimozato, O. et al. (2006) Immunology 117:22.
- Russell, T.D. et al. (2003) J. Immunol. 171:6866.
Product Datasheets
Citation for Mouse IL-12 R beta 1 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction
Authors: J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss
The Journal of Biological Chemistry, 2021-10-09;0(0):101295.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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