Mouse IL-17RA/IL-17R Antibody

Catalog #: MAB4481
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4481
MAB4481-SP

Detection of IL‑17 RA/IL‑17 R in EL-4 Mouse Cell Line by Flow Cytometry.

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Citations (7)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-17RA/IL-17R Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL-17 RA, recombinant mouse (rm) IL-17B R, rmIL-17 RD, or rmIL-17 RC is observed. In Western blots, less than 10% cross‑reactivity with recombinant human (rh) IL-17 RA or rhIL-17B R is observed.

Source

Monoclonal Rat IgG_2A Clone # 657603

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RA/IL‑17 R
Ser32-Trp322
Accession # Q60943

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize IL‑17-induced IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. Yao, Z. et al. (1995) Immunity 3:811.The Neutralization Dose (ND₅₀) is typically 0.2-0.8 μg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑17.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑17 RA/IL‑17 R in EL-4 Mouse Cell Line by Flow Cytometry. EL-4 mouse lymphoblast cell line was stained with Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB4481, filled histo-gram) or isotype control antibody (Catalog # MAB006, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Neutralization

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IL-6 secretion Induced by IL‑17 and Neutralization by Mouse IL‑17 RA/IL‑17 R Antibody. Recombinant Mouse IL-17 (Catalog # 421-ML) induces IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine kit (Catalog # M6000B). IL-6 secretion elicited by Recombinant Mouse IL-17 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-17 RA/IL-17 R Monoclonal Antibody (Catalog # MAB4481). The ND₅₀ is typically 0.2-0.8 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RA/IL-17R

IL-17 receptor alpha subunit (IL-17 RA), also known as IL-17 R, is a widely expressed 120 kDa glycosylated type I transmembrane protein that plays a central role in inflammatory responses. IL‑17 RA associates with IL-17 RC to form a signaling receptor complex for IL-17 and IL-17F. IL-17 RA ligation promotes T cell activation and the production of IL-6, G‑CSF, SCF, and multiple proinflammatory chemokines. Within the ECD, mouse IL-17 RA shares 84% and 72% aa sequence identity with rat and human IL17 RA, respectively.

Long Name

Interleukin 17 Receptor

Entrez Gene IDs

23765 (Human); 16172 (Mouse); 312679 (Rat); 486759 (Canine); 101925679 (Cynomolgus Monkey)

Alternate Names

CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-17RA/IL-17R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology
Authors: D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber
Nature Immunology, 2022-10-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death
Authors: T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek
Cell. Signal., 2020-06-15;73(0):109690.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: Neutralization, Western Blot
Interleukin-17A participates in podocyte injury by inducing IL-1? secretion through ROS-NLRP3 inflammasome-caspase-1 pathway
Authors: J Yan, Y Li, H Yang, L Zhang, B Yang, M Wang, Q Li
Scand. J. Immunol., 2018-03-05;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Interleukin-17A Promotes Lung Tumor Progression Through Neutrophil Attraction to Tumor Sites and Mediating Resistance to PD-1 Blockade
Authors: EA Akbay, S Koyama, Y Liu, R Dries, LE Bufe, M Silkes, MM Alam, DM Magee, R Jones, M Jinushi, M Kulkarni, J Carretero, X Wang, T Warner-Hat, JD Cavanaugh, A Osa, A Kumanogoh, GJ Freeman, MM Awad, DC Christiani, R Bueno, PS Hammerman, G Dranoff, KK Wong
J Thorac Oncol, 2017-05-06;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
Blocking IL-17A Alleviates Diabetic Retinopathy in Rodents
Authors: AW Qiu, QH Liu, JL Wang
Cell. Physiol. Biochem, 2017-02-21;41(3):960-972.
Species: Mouse, Rat
Sample Types: In Vivo, Whole Cells
Applications: Neutralization
Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function
Proc Natl Acad Sci USA, 2016-09-26;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Galectin-3 negatively regulates dendritic cell production of IL-23/IL-17-axis cytokines in infection by Histoplasma capsulatum.
Authors: Wu S, Yu J, Liu F, Miaw S, Wu-Hsieh B
J Immunol, 2013-03-01;190(7):3427-37.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization