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Mouse IL-20 Antibody

Catalog #: MAB1204
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MAB1204
MAB1204-SP
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Citations (1)
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Mouse IL-20 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-20 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human IL-20 is observed.
Source
Monoclonal Rat IgG2B Clone # 176005
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL‑20
Leu25-Leu176
Accession # Q9JKV9.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑20 (Catalog # 1204-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-20

Mouse Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences with a signal sequence and amphipathic helices found in helical cytokines. Based on the human molecule, mouse IL-20 was discovered in a skin library. Mouse IL-20 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 are 77% aa identical in the mature segment. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first complex is composed of IL-20 R alpha and IL‑20 R beta. The second complex is composed of IL-22 R and IL-20 R beta. Whereas the IL-22 R/IL‑20 R beta complex is shared with IL-24/mda-7, the IL‑20 R alpha /IL‑20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of pro-inflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1-6).

References
  1. Blumberg, H. et al. (2001) Cell 104:9.
  2. Liu, L. et al. (2003) Blood 102:3206.
  3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.
  4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  5. Dumoutier, L. (2001) J. Immunol. 167:3545. 
  6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.
Long Name
Interleukin 20
Entrez Gene IDs
50604 (Human); 58181 (Mouse)
Alternate Names
Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907

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Citation for Mouse IL-20 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A novel transcript of mouse interleukin-20 receptor acts on glomerular mesangial cells as an aggravating factor in lupus nephritis.
    Authors: Wei CC, Chang MS
    Genes Immun., 2008-09-04;9(8):668-79.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot

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