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Mouse IL-21 Antibody

Catalog #: AF594
23 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF594
AF594-SP
IL‑21 in Mouse Splenocytes.
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Citations (23)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-21 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-21 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL-15, and recombinant human IL-21 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML)
Pro25-Ser146
Accession # Q9ES17
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑21 (Catalog # 594-ML)
Immunocytochemistry
5-15 µg/mL
See below

Mouse IL-21 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL‑21 Biotinylated Antibody (Catalog # BAF594)

Standard: Recombinant Mouse IL-21 Protein (Catalog # 594-ML)

Neutralization
Measured by its ability to neutralize IL‑21-induced proliferation in the NK-92 human natural killer lymphoma cell line. The Neutralization Dose (ND50) is typically 0.2-2 µg/mL in the presence of 10 ng/mL Recombinant Mouse IL‑21.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL‑21 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

IL‑21 in Mouse Splenocytes. IL‑21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization View Larger

Cell Proliferation Induced by IL-21 and Neutralization by Mouse IL-21 Antibody. Recombinant Mouse IL-21 (Catalog # 594-ML) stimulates proliferation in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-21 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594). The ND50 is typically 0.2-2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-21

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid sequence identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma c) chain appears to play a role in IL-21 R signaling. The IL‑21/IL‑21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

References
  1. Parrish-Novak, et al. (2000) Nature 408:57.
  2. Ozaki, K. et al. (2000) PNAS 97:11439.
  3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
  4. Asao, H. et al. (2001) J. Immunol. 167:1.
Long Name
Interleukin 21
Entrez Gene IDs
59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)
Alternate Names
CVID11; IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform; Za11

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Citations for Mouse IL-21 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Treatment with a Lactococcus lactis that chromosomally express E. coli cfaI mitigates salivary flow loss in a Sjögren's syndrome-like disease
    Authors: Akgul, A;Freguia, CF;Maddaloni, M;Hoffman, C;Voigt, A;Nguyen, CQ;Fanger, NA;Fanger, GR;Pascual, DW;
    Scientific reports
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. Lung regeneration with rat fetal lung implantation and promotion of alveolar stem cell differentiation by corticosteroids
    Authors: Matsumoto D, Toba H, Kenzaki K et al.
    Regen Ther
  3. CHD7 variants associated with hearing loss and enlargement of the vestibular aqueduct
    Authors: Roux I, Fenollar-Ferrer C, Lee HJ et al.
    Hum Genet
  4. Astroglial Hmgb1 regulates postnatal astrocyte morphogenesis and cerebrovascular maturation
    Authors: Freitas-Andrade, M;Comin, CH;Van Dyken, P;Ouellette, J;Raman-Nair, J;Blakeley, N;Liu, QY;Leclerc, S;Pan, Y;Liu, Z;Carrier, M;Thakur, K;Savard, A;Rurak, GM;Tremblay, MÈ;Salmaso, N;da F Costa, L;Coppola, G;Lacoste, B;
    Nature communications
  5. Siglec-6 mediates the uptake of extracellular vesicles through a noncanonical glycolipid binding pocket
    Authors: EN Schmidt, D Lamprinaki, KA McCord, M Joe, M Sojitra, A Waldow, J Nguyen, J Monyror, EN Kitova, F Mozaneh, XY Guo, J Jung, JR Enterina, GC Daskhan, L Han, AR Krysler, CR Cromwell, BP Hubbard, LJ West, M Kulka, S Sipione, JS Klassen, R Derda, TL Lowary, LK Mahal, MR Riddell, MS Macauley
    Nature Communications, 2023-04-22;14(1):2327.
  6. Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4-pRB Axis in Fibroblasts at the Invasive Tumor Edge
    Authors: Bouchard G, Garcia-Marques FJ, Karacosta LG et al.
    Cancer Research
  7. Monocyte Gene and Molecular Expression Profiles Suggest Distinct Effector and Regulatory Functions in Beninese HIV Highly Exposed Seronegative Female Commercial Sex Workers
    Authors: L Blondin-La, L Fourcade, A Modica, M Aranguren, N de Montign, AC Labbé, M Alary, F Guédou, J Poudrier, M Roger
    Viruses, 2022-02-10;14(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Mechanical Stimulation-Induced Calcium Signaling by Piezo1 Channel Activation in Human Odontoblast Reduces Dentin Mineralization
    Authors: Matsunaga M, Kimura M, Ouchi T et al.
    Frontiers in Physiology
  9. Characterization of IL-21-expressing recombinant hepatitis B virus (HBV) as a therapeutic agent targeting persisting HBV infection
    Authors: Z Shen, J Wu, Z Gao, J Wang, H Zhu, R Mao, X Wang, J Zhang, Y Xie, J Liu
    Theranostics, 2020-04-25;10(12):5600-5612.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Regulation of Transcription Factor E2-2 in Human Plasmacytoid Dendritic Cells by Monocyte-Derived TNF&alpha
    Authors: HK Dewald, HJ Hurley, P Fitzgerald
    Viruses, 2020-01-31;12(2):.
    Species: Human
    Sample Types: Whole Blood
    Applications: Flow Cytometry
  11. Chronic myelogenous leukemia stem cells require cell-autonomous pleiotrophin signaling
    Authors: HA Himburg, M Roos, T Fang, Y Zhang, CM Termini, L Schlussel, MM Kim, A Pang, J Kan, L Zhao, H Suh, JP Sasine, G Sapparapu, PM Bowers, G Schiller, JP Chute
    J. Clin. Invest., 2020-01-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  12. Prostaglandin E2 inhibits Tr1 cell differentiation through suppression of c-Maf
    Authors: KM Hooper, W Kong, D Ganea
    PLoS ONE, 2017-06-12;12(6):e0179184.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Intestinal microbiota link lymphopenia to murine autoimmunity via PD-1(+)CXCR5(-/dim) B-helper T cell induction
    Authors: T Eri, K Kawahata, T Kanzaki, M Imamura, K Michishita, L Akahira, E Bannai, N Yoshikawa, Y Kimura, T Satoh, S Uematsu, H Tanaka, K Yamamoto
    Sci Rep, 2017-04-26;7(0):46037.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015-07-16;290(37):22338-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  15. STAT3-dependent IL-21 production from T helper cells regulates hematopoietic progenitor cell homeostasis.
    Authors: Kaplan MH, Glosson NL, Stritesky GL, Yeh N, Kinzfogl J, Rohrabaugh SL, Goswami R, Pham D, Levy DE, Brutkiewicz RR, Blum JS, Cooper S, Hangoc G, Broxmeyer HE
    Blood, 2011-04-19;117(23):6198-201.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  16. Interleukin-21 is critically required in autoimmune and allogeneic responses to islet tissue in murine models.
    Authors: McGuire HM, Walters S, Vogelzang A
    Diabetes, 2011-03-01;60(3):867-75.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).
    Authors: Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW
    PLoS ONE, 2010-01-14;5(1):e8720.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  18. Cytokine-secreting follicular T cells shape the antibody repertoire.
    Authors: Reinhardt RL, Liang HE, Locksley RM
    Nat. Immunol., 2009-03-01;10(4):385-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  19. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008-03-19;112(2):362-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  20. IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner.
    Authors: Wei L, Laurence A, Elias KM, O'Shea JJ
    J. Biol. Chem., 2007-09-20;282(48):34605-10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Expression and one-step purification of bovine interleukin-21 (IL-21) in silkworms using a hybrid baculovirus expression system.
    Authors: Muneta Y, Nagaya H, Minagawa Y, Enomoto C, Matsumoto S, Mori Y
    Biotechnol. Lett., 2004-09-01;26(18):1453-8.
    Species: Bovine
    Sample Types: Recombinant Protein
    Applications: Western Blot
  22. IL-21 induces the functional maturation of murine NK cells.
    Authors: Brady J, Hayakawa Y, Smyth MJ, Nutt SL
    J. Immunol., 2004-02-15;172(4):2048-58.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  23. IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
    Authors: Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S
    J. Immunol., 2004-02-01;172(3):1540-7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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