Home / IL-4 / Mouse IL-4 Biotinylated Antibody

Mouse IL-4 Biotinylated Antibody

Catalog #: BAF404
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAF404
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-4 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-4 in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-4 (R&D Systems, Catalog # 404-ML)
His23-Ser140
Accession # P07750
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Purity
Antigen Affinity-purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑4 (Catalog # 404-ML)

Mouse IL-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IL‑4 Antibody (Catalog # MAB404)

Capture Reagent: Mouse IL‑4 Antibody (Catalog # MAB404R)

Standard: Recombinant Mouse IL-4 Protein (Catalog # 404-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa - 18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1 - 4). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (5). Mouse IL-4 is synthesized with a 24 aa signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (6 - 8). IL-4 exerts its effects through two receptor complexes (9, 10). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in mouse B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (11 - 14). IL-4 plays a dominant role in the development of allergic inflammation and asthma (13, 15).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Lee, F. et al. (1986) Proc. Natl. Acad. Sci. 83:2061.
  4. Noma, Y. et al. (1986) Nature 319:640.
  5. Redfield, C. et al. (1991) Biochemistry 30:11029.
  6. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  7. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  8. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  9. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  10. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  11. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  12. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  13. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  14. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  15. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse IL-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Depletion of Foxp3+ regulatory T cells augments CD4+ T cell immune responses in atherosclerosis-prone hypercholesterolemic mice
    Authors: K Kasahara, N Sasaki, HZ Amin, T Tanaka, S Horibe, T Yamashita, KI Hirata, Y Rikitake
    Heliyon, 2022-07-19;8(7):e09981.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  2. Dual vaccination against IL-4 and IL-13 protects against chronic allergic asthma in mice
    Authors: E Conde, R Bertrand, B Balbino, J Bonnefoy, J Stackowicz, N Caillot, F Colaone, S Hamdi, R Houmadi, A Loste, JBJ Kamphuis, F Huetz, L Guillemina, N Gaudenzio, A Mougel, D Hardy, JN Snouwaert, BH Koller, V Serra, P Bruhns, G Grouard-Vo, LL Reber
    Nature Communications, 2021-05-11;12(1):2574.
    Species: Mouse
    Sample Types: Recombinant Proteins
    Applications: ELISA Detection
  3. Interleukin (IL)-25 suppresses IL-22-induced osteoclastogenesis in rheumatoid arthritis via STAT3 and p38 MAPK/I&kappaB&alpha pathway
    Authors: HK Min, JY Won, BM Kim, KA Lee, SJ Lee, SH Lee, HR Kim, KW Kim
    Arthritis Research & Therapy, 2020-09-23;22(1):222.
    Species: Human
    Sample Types: Synovial Fluid
  4. Characterization of the immune response elicited by the vaccinia virus L3 protein delivered as naked DNA
    Authors: M Ramírez, S Santos, O Martínez, R Rodríguez, E Miranda, WD Ramos-Pere, M Otero
    Vaccine, 2018-03-07;36(15):2049-2055.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA Detection
  5. Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells
    Authors: W Dawicki, C Li, J Town, X Zhang, JR Gordon
    J. Allergy Clin. Immunol., 2016-10-26;139(5):1608-1620.e3.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  6. CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction.
    Authors: Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C
    Eur. J. Immunol., 2012-05-01;42(5):1250-60.
    Species: Mouse
    Sample Types: Pleural Lavage Fluid
    Applications: ELISA Development
  7. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  8. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009-03-13;11(4):524-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  9. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008-04-03;125(2):272-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  10. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006-06-01;168(6):1940-50.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  11. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
    Authors: Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
    Am. J. Pathol., 2002-04-01;160(4):1353-60.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse IL-4 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse IL-4 Biotinylated Antibody and earn rewards!

Have you used Mouse IL-4 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review