Mouse IL-6R alpha Antibody

Catalog #: MAB1830
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1830
MAB1830-SP

All IL-6R alpha Antibodies

Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-6R alpha Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑6 R alpha in direct ELISAs. In direct ELISAs, no cross‑reactivity with recombinant human IL-6 R alpha is observed.

Source

Monoclonal Rat IgG_2A Clone # 255821

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-6 R alpha
Leu20-Glu357
Accession # P22272

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

Mouse CD3⁺ splenocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

Interleukin 6 (IL-6) is a multifunctional cytokine that exerts its activities by binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa ligand binding subunit (IL-6 R alpha /CD126) and a 130 kDa nonligand-binding signal-transducing subunit (gp130/CD130) (1‑4). Mature mouse IL-6 R alpha is a type I transmembrane protein of 460 amino acids (aa) that contains a 345 aa extracellular ligand binding domain, a 21 aa transmembrane region, and a 75 aa cytoplasmic segment (2). The extracellular segment contains an Ig-like and a fibronectin-type III domain, plus a membrane proximal WSxWS motif. In their extracellular regions, mouse IL‑6 R alpha shares 89%, 51% and 50% aa identity with rat, human and porcine IL‑6 R alpha, respectively. Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). Two soluble receptor isoforms that arise either from proteolytic cleavage of the membrane-bound IL‑6 R alpha, or by alternative mRNA splicing (reported only in human) have been described (6, 7). Soluble IL-6 R alpha binds IL-6 with an affinity similar to that of the membrane-bound IL-6 R alpha. More importantly, the soluble IL-6 R alpha /IL-6 complex is capable of interacting with the membrane-bound gp130 to activate cells that lack an integral membrane IL-6 R alpha. It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn’s disease (6, 7).

References

Yamasaki, K. et al. (1988) Science 241:825.
Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
Hibi, M. et al. (1990) Cell 63:1149.
Saito, M. et al. (1992) J. Immunol. 148:4066.
Novick, D. et al. (1989) J. Exp. Med. 170:1409.
Jones, S.A. et al. (2001) FASEB J. 15:43.
Jones, S.A. and S. Rose-John (2002) Biochim. Biophys. Acta 1592:251.

Long Name

Interleukin 6 Receptor alpha

Entrez Gene IDs

3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)

Alternate Names

CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IL-6R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Autocrine interleukin-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca(2+) channels.
Authors: Ke F, Zhang L, Liu Z, Liu J, Yan S, Xu Z, Bai J, Zhu H, Lou F, Wang H, Shi Y, Jiang Y, Su B, Wang H
Stem Cells, 2014-10-01;32(10):2799-810.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC-P
Ifitm3 limits the severity of acute influenza in mice.
Authors: Bailey, Charles, Huang, I-Chueh, Kam, Christin, Farzan, Michael
PLoS Pathog, 2012-09-06;8(9):e1002909.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Essential roles of IL-6 trans-signaling in colonic epithelial cells, induced by the IL-6/soluble-IL-6 receptor derived from lamina propria macrophages, on the development of colitis-associated premalignant cancer in a murine model.
Authors: Matsumoto S, Hara T, Mitsuyama K, Yamamoto M, Tsuruta O, Sata M, Scheller J, Rose-John S, Kado S, Takada T
J. Immunol., 2009-12-30;184(3):1543-51.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr