Mouse IL-7 Antibody

Catalog #: AB-407-NA
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AB-407-NA

Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody.

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Citations (3)

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Mouse IL-7 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human IL-7 is observed.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse IL-7 (R&D Systems, Catalog # 407-ML)
Glu26-Ile154
Accession # Q544C8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑7 (Catalog # 407-ML)

Neutralization

Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 1.5 ng/mL Recombinant Mouse IL‑7.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑7 and Neutralization by Mouse IL‑7 Antibody. Recombinant Mouse IL‑7 (Catalog # 407-ML) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑7 Polyclonal Antibody (Catalog # AB-407-NA). The ND₅₀ is typically 0.5-1.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7, previously known as pre-B cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4⁺and CD8⁺ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumorocidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues.

Mouse IL-7 cDNA encodes a precursor protein of 154 amino residues containing a 25 amino acid residue signal peptide. Human IL-7 has approximately 65% amino acid sequence identity with mouse IL-7 and both proteins exhibit cross-species activity.

IL-7 bioactivities are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells, and bone marrow cells.

Long Name

Interleukin 7

Entrez Gene IDs

3574 (Human); 16196 (Mouse); 25647 (Rat)

Alternate Names

IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Mouse IL-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Interleukin-7 and -15 maintain pathogenic memory Th17 cells in autoimmunity
Authors: Yihe Chen
J. Autoimmun, 2016-11-26;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease.
Authors: Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ
Nat. Med., 2010-01-10;16(2):191-7.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization