Mouse IL-7R alpha/CD127 Antibody

Catalog #: MAB7473 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB7473
MAB7473-SP

Detection of IL‑7 R alpha /CD127 in Mouse Splenocytes by Flow Cytometry.

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Mouse IL-7R alpha/CD127 Antibody Summary

Species Reactivity

Mouse

Specificity

Stains mouse IL‑7 R alpha /CD127 transfectants but not irrelevant transfectants in flow cytometry.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1140A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NSO mouse myeloma cell line transfected with mouse IL‑7 R alpha /CD127
Glu21-Asp239
Accession # P16872

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑7 R alpha /CD127 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rabbit Anti-Mouse IL-7 Ra/CD127 Monoclonal Antibody (Catalog # MAB7473) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature mouse IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type III domain and a WSxWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Within the ECD, mouse IL-7 R alpha shares 67% and 79% aa sequence identity with human and rat IL‑7 R alpha, respectively. IL-7 R alpha associates with the common gamma chain ( gamma _c) to form the functional high affinity IL-7 receptor complex (4). The gamma _c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL-7 show cross-species activity through the IL-7 receptor (3, 5). IL‑7 R alpha is expressed on double negative (CD4^-CD8^-) and CD4⁺ or CD8⁺ single positive T cells as well as on CD8⁺ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL‑7 R alpha is critical for both T cell and B cell lineage development (8). In human it is required for T cell but not for B cell development (9). IL‑7 induces the down regulation and shedding of cell surface IL-7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL‑7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL‑7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References

Mazzucchelli, R. and S.K. Durum, 2007, Nat. Rev. Immunol. 7:144.
Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.
Goodwin, R.G. et al. (1990) Cell 60:941.
Noguchi, M. et al. (1993) Science 262:1877.
Barata, J.T. et al. (2006), Exp. Hematol. 34:1133.
Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
Park, L.S. et al. (2000) J. Exp. Med. 192:659.
Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Reche, P.A. et al. (2001) J. Immunol. 167:336.
Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
Jahn, T. et al. (2007) Blood 110:1840.
Lai, L. et al. (2006) Blood 107:1776.

Long Name

Interleukin 7 Receptor alpha

Entrez Gene IDs

3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)

Alternate Names

CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha