Mouse IL-9 Antibody

Catalog #: AB-409-NA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AB-409-NA

Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody.

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Mouse IL-9 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human IL-9 is observed. Neutralizes the biological activity of recombinant mouse IL-9. It will not neutralize the biological activity of recombinant human IL-9.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑9
Gln19-Pro144
Accession # P15247

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse IL‑9 (Catalog # 409-ML)

Neutralization

Measured by its ability to neutralize IL‑9-induced proliferation in the TS1 mouse helper T cell line. Uyttenhove, C. et al. (1988) Proc. Natl. Acad. Sci. 85:6934. The Neutralization Dose (ND₅₀) is typically 3-18 µg/mL in the presence of 0.05 ng/mL Recombinant Mouse IL‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑9 and Neutralization by Mouse IL‑9 Antibody. Recombinant Mouse IL-9 (Catalog # 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-9 Polyclonal Antibody (Catalog # AB-409-NA). The ND₅₀ is typically 3-18 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9

Mouse IL-9 was originally identified as a T cell-derived T cell growth factor III/P40 which could support the long term growth of certain mouse T helper clones in the absence of antigen or antigen-presenting cells. IL-9 can also prolong the in vitro survival of other T cell clones as well as potentiate the IL-2 dependent proliferation of mouse fetal thymocytes. However, this cytokine has no growth-stimulating activity on mouse cytolytic T cell clones or fresh T cells. In addition to its activities on T cells, mouse IL-9 also has mast cell enhancing activity (MEA) and can enhance the mIL-3- or mIL-4-dependent proliferation of mouse bone marrow-derived mast cells. Furthermore, IL-9 will synergize with erythropoietin to support erythroid colony formation in vitro.

The gene for mIL-9 has been mapped to mouse chromosome 13. The mouse IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Mouse IL-9 contains four potential N-linked glycosylation sites and the native mIL-9 is a highly glycosylated protein.

Human IL-9 was independently cloned as a novel growth factor which is mitogenic for the human megakaryoblastic leukemic cell line, M07e. Human and mouse IL-9 share 56% and 67% homology at the amino acid and nucleotide levels, respectively. Although mouse IL-9 is active on human cells, human IL-9 is not active on mouse cells.

References

Renauld, J.E. et al. (1995) J. Leukoc. Biol 57:303.

Long Name

Interleukin 9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Alternate Names

Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40