Mouse Integrin alpha 2b/CD41 Antibody

Catalog #: MAB41181
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB41181
MAB41181-SP

Integrin alpha 2b/CD41 in Mouse Bone Marrow Cells.

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Mouse Integrin alpha 2b/CD41 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha 2b/CD41 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 386629

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 2b/CD41
Leu32-Arg988
Accession # NP_034705

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Mouse platelets

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Integrin alpha 2b/CD41 in Mouse Bone Marrow Cells. Integrin a2b/CD41 was detected in immersion fixed mouse bone marrow cells using Rat Anti-Mouse Integrin a2b/CD41 Monoclonal Antibody (Catalog # MAB41181) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL014) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 2b/CD41

Integrin alpha 2b, (ITGA2b) also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114 kDa and 22 kDa) during transit through the Golgi. ITGA2b associates with integrin beta 3 to form complexes that interact with fibrinogen, von Willebrand factor, fibronectin, and vitronectin. ITGA2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, mouse ITGA2b shares 81% and 89% amino acid sequence identity with human and rat ITGA2b, respectively.

Entrez Gene IDs

3674 (Human); 16399 (Mouse); 29350 (Rat); 102119413 (Cynomolgus Monkey)

Alternate Names

CD41 antigen; CD41; CD41BHPA3; GP2B; GP2Bintegrin alpha-IIb; GPalpha IIb; GPIIb; GTA; HPA3; Integrin alpha 2b; integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41); integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41B); ITGA2b; ITGAB; platelet fibrinogen receptor, alpha subunit; Platelet membrane glycoprotein IIb; platelet-specific antigen BAK

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse Integrin alpha 2b/CD41 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Cysteinyl leukotriene receptor 2 drives lung immunopathology through a platelet and high mobility box 1-dependent mechanism
Authors: T Liu, NA Barrett, Y Kanaoka, K Buchheit, TM Laidlaw, D Garofalo, J Lai, HR Katz, C Feng, JA Boyce
Mucosal Immunol, 2019-01-21;0(0):.
Species: Mouse
Sample Types: Platelet-Rich Plasma
Applications: Flow Cytometry