Mouse Integrin alpha 5/CD49e Antibody

Catalog #: AF1754
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1754
AF1754-SP

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin alpha 5/CD49e Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha 5/CD49e in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human (rh) Integrin alpha 5 is observed, 15% cross‑reactivity with rhIntegrin alpha 2 and rhIntegrin alpha M is observed, and 5% cross‑reactivity with rhIntegrin alpha E is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 5/CD49e
Phe45-Asn (Gln68Glu)
Accession # P11688

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Integrin alpha 5/CD49e

Flow Cytometry

0.25 µg/10⁶ cells

B16‑F1 mouse melanoma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

The alpha 5 subunit, also known as CD49e and VLA-5 alpha subunit, forms a heterodimer with the Integrin beta 1 subunit (CD29). Integrin alpha 5 beta 1 is a receptor for fibronectin and neural adhesion molecule L1.

Entrez Gene IDs

3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)

Alternate Names

CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Integrin alpha 5/CD49e Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Direct regulation of osteocytic connexin 43 hemichannels through AKT kinase activated by mechanical stimulation.
Authors: Batra N, Riquelme M, Burra S, Kar R, Gu S, Jiang J
J Biol Chem, 2014-02-22;289(15):10582-91.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot
Mechanical stress-activated integrin alpha5beta1 induces opening of connexin 43 hemichannels.
Authors: Batra N, Burra S, Siller-Jackson AJ, Gu S, Xia X, Weber GF, DeSimone D, Bonewald LF, Lafer EM, Sprague E, Schwartz MA, Jiang JX
Proc. Natl. Acad. Sci. U.S.A., 2012-02-13;109(9):3359-64.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC