Mouse Integrin alpha 8 Biotinylated Antibody Summary
Phe38-Phe1007
Accession # A2ARA8
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Integrin alpha 8 in 4T1 Mouse Cell Line. Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse Integrin a8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Mouse Integrin alpha 8 by Western Blot. Western blot shows lysates of Mouse Lung and Rat Lung. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Integrin alpha 8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4076) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Integrin alpha 8 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 8
Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin alpha 8 protein, respectively.
Product Datasheets
Citations for Mouse Integrin alpha 8 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis
Authors: AE Jarmas, EW Brunskill, P Chaturvedi, N Salomonis, R Kopan
Nature Communications, 2021-11-03;12(1):6332.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
Authors: Y Murakami, H Naganuma, S Tanigawa, T Fujimori, M Eto, R Nishinakam
Sci Rep, 2019-02-04;9(1):1172.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry -
Regenerative potential of induced pluripotent stem cells derived from patients undergoing haemodialysis in kidney regeneration
Authors: S Tajiri, S Yamanaka, T Fujimoto, K Matsumoto, A Taguchi, R Nishinakam, HJ Okano, T Yokoo
Sci Rep, 2018-10-08;8(1):14919.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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