Mouse Integrin beta 4/CD104 Antibody

Catalog #: AF4054
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4054
AF4054-SP

Detection of Mouse Integrin beta 4/CD104 by Western Blot.

1 Image

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin beta 4/CD104 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Integin beta 4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 4/CD104
Asn29-Cys707
Accession # NP_001005608

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse Integrin beta 4/CD104 by Western Blot. Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Integrin beta 4/CD104 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4054) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Three specific bands were detected for Integrin beta 4/CD104 at approximately 150-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 4/CD104

Integrin beta 4 (also GP150 and CD104) is a 150‑180 kDa member of the integrin beta family of molecules. It is found on keratinocytes, Schwann cells and perineural fibroblasts, and participates in the formation of epithelial hemidesmosomes by forming noncovalent heterodimers with Integrin alpha 6. Mature mouse Integrin beta 4 is a type I transmembrane glycoprotein that is 1774 amino acids (aa) in length. It contains a 683 aa extracellular domain (aa 29‑711) and a large 1068 aa cytoplasmic region. Three variant splice forms may exist. One shows a GlyGluHis insertion after Leu1103, a second shows a deletion of aa 1447‑1499, and a third shows the same deletion accompanied by an insertion of 65 aa after Thr1367. Over aa 29‑711, mouse beta 4 shares 88% and 96% aa identity with human and rat beta 4, respectively.

Entrez Gene IDs

3691 (Human); 192897 (Mouse); 25724 (Rat)

Alternate Names

CD104 antigen; CD104; GP150; Integrin beta 4; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Integrin beta 4/CD104 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Integrin alpha6 maintains the structural integrity of the kidney collecting system
Authors: Olga M Viquez
Matrix Biol, 2016-12-30;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: Flow Cytometry, Western Blot