Home / Ki67/MKI67 / Mouse Ki67/MKI67 Antibody

Mouse Ki67/MKI67 Antibody

Catalog #: AF7649
17 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7649
AF7649-SP
Ki67/MKI67 in NIH‑3T3 Mouse Cell Line.
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Citations (17)
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Mouse Ki67/MKI67 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Ki67/MKI67 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Ki67/MKI67 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Ki67/MKI67
Asn3053-Ser3177
Accession # NP_001074586
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Ki67/MKI67 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Ki67/MKI67 in NIH‑3T3 Mouse Cell Line. Ki67/MKI67 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Sheep Anti-Mouse Ki67/MKI67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7649) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ki67/MKI67

MKI67 (also Ki67 and TSG126) is a 350-370 kDa nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins. Ki67 was originally recognized as an antigen associated with the monoclonal Ki67 antibody raised against Hodgkin's lymphoma nuclear material. Ki67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle. Thus, MKI67 qualifies as a cell proliferation marker. Functionally, Ki67 is known to interact with 160 kDa Hklp2, a protein that promotes centrosome separation and spindle bipolarity. It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis. Mouse MKI67 is 3177 amino acids (aa) in length. It contains one FHA domain (aa 8-101), followed by sixteen 120 aa repeats (aa 993-2872). There are two potential isoform variants. One isoform shows a 19 aa substitution for 1120-3177, while a second isoform contains a deletion of aa 1169-1409. Over aa 3053-3177, mouse Ki67 shares 46% and 74% aa sequence identity with the human and rat orthologs to Ki67, respectively.

Long Name
Antigen Identified by Monoclonal Antibody Ki67
Entrez Gene IDs
4288 (Human); 17345 (Mouse); 291234 (Rat)
Alternate Names
antigen identified by monoclonal Ki-67; Antigen Ki67; antigen KI-67; Ki67; Ki-67; KIA; Marker Of Proliferation Ki-67; MIB-; MIB-1; MKI67; PPP1R105; Proliferation Marker Protein Ki-67; proliferation-related Ki-67 antigen; Protein Phosphatase 1; Regulatory Subunit 105; TSG126

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Citations for Mouse Ki67/MKI67 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Systemic delivery of glycosylated-PEG-masked oncolytic virus enhances targeting of antitumor immuno-virotherapy and modulates T and NK cell infiltration
    Authors: Liang, Y;Wang, B;Chen, Q;Fu, X;Jiang, C;Lin, Z;Zhuang, Q;Zeng, Y;Liu, X;Zhang, D;
    Theranostics
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Atoh1 drives the heterogeneity of the pontine nuclei neurons and promotes their differentiation
    Authors: Wu, SR;Butts, JC;Caudill, MS;Revelli, JP;Dhindsa, RS;Durham, MA;Zoghbi, HY;
    Science advances
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins
    Authors: M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu
    bioRxiv : the preprint server for biology, 2023-04-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Human Bone Marrow-Derived Mesenchymal Stromal Cells Reduce the Severity of Experimental Necrotizing Enterocolitis in a Concentration-Dependent Manner
    Authors: L Provitera, A Tomaselli, G Raffaeli, S Crippa, C Arribas, I Amodeo, S Gulden, GS Amelio, V Cortesi, F Manzoni, G Cervellini, J Cerasani, C Menis, N Pesenti, M Tripodi, L Santi, M Maggioni, C Lonati, S Oldoni, F Algieri, F Garrido, ME Bernardo, F Mosca, G Cavallaro
    Cells, 2023-02-27;12(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Hydrogel oxygen reservoirs increase functional integration of neural stem cell grafts by meeting metabolic demands
    Authors: Y Wang, ER Zoneff, JW Thomas, N Hong, LL Tan, DJ McGillivra, AW Perriman, KCL Law, LH Thompson, N Moriarty, CL Parish, RJ Williams, CJ Jackson, DR Nisbet
    Nature Communications, 2023-01-28;14(1):457.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Role of ERK signaling in bladder urothelium in response to cyclophosphamide injury
    Authors: ST Narla, JL Duara, DS Bushnell, M Nouraie, J Holden, K Pfister, PC Lucas, S Sims-Lucas, CM Bates
    Physiological Reports, 2022-07-01;10(14):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Durability of and role of AKT in FGF7p urothelial protection against cyclophosphamide
    Authors: ST Narla, L Rice, D Ostrov, DS Bushnell, JL Duara, CM Bates
    Physiological Reports, 2022-06-01;10(12):e15358.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Hedgehog-responsive PDGFRa(+) fibroblasts maintain a unique pool of alveolar epithelial progenitor cells during alveologenesis
    Authors: F Gao, C Li, S Danopoulos, D Al Alam, N Peinado, S Webster, Z Borok, GA Kohbodi, S Bellusci, P Minoo
    Cell Reports, 2022-04-05;39(1):110608.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke
    Authors: J Wang, R Hao, T Jiang, X Guo, F Zhou, L Cao, F Gao, G Wang, J Wang, K Ning, C Zhong, X Chen, Y Huang, J Xu, S Gao
    Cell & bioscience, 2022-04-04;12(1):40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. FGF7 peptide (FGF7p) mimetic mitigates bladder urothelial injury from cyclophosphamide
    Authors: ST Narla, L Rice, D Ostrov, SG Swarts, DW Siemann, DS Bushnell, JG Holden, JL Duara, CM Bates
    Physiological Reports, 2022-04-01;10(7):e15241.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Growth differentiation factor 1-induced tumour plasticity provides a therapeutic window for immunotherapy in hepatocellular carcinoma
    Authors: W Cheng, HL Li, SY Xi, XF Zhang, Y Zhu, Le Xing, YX Mo, MM Li, FE Kong, WJ Zhu, XG Chen, HQ Cui, ZM Cao, YF Gong, YQ Tang, Y Zhang, XY Guan, NF Ma, M Liu
    Nature Communications, 2021-12-08;12(1):7142.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  12. Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression
    Authors: JW Opzoomer, JE Anstee, I Dean, EJ Hill, I Bouybayoun, J Caron, T Muliaditan, P Gordon, D Sosnowska, R Nuamah, SE Pinder, T Ng, F Dazzi, S Kordasti, DR Withers, T Lawrence, JN Arnold
    Science Advances, 2021-11-03;7(45):eabg9518.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Reactivity and increased proliferation of NG2 cells following central nervous system infection with Theiler's murine encephalomyelitis virus
    Authors: LA Bell, GJ Wallis, KS Wilcox
    Journal of Neuroinflammation, 2020-12-03;17(1):369.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. The aging skin microenvironment dictates stem cell behavior
    Authors: Y Ge, Y Miao, S Gur-Cohen, N Gomez, H Yang, M Nikolova, L Polak, Y Hu, A Verma, O Elemento, JG Krueger, E Fuchs
    Proc. Natl. Acad. Sci. U.S.A., 2020-02-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Amino Acid Transporter Slc38a5 Controls Glucagon Receptor Inhibition-Induced Pancreatic ? Cell Hyperplasia in Mice
    Authors: J Kim, H Okamoto, Z Huang, G Anguiano, S Chen, Q Liu, K Cavino, Y Xin, E Na, R Hamid, J Lee, B Zambrowicz, R Unger, AJ Murphy, Y Xu, GD Yancopoulo, WH Li, J Gromada
    Cell Metab., 2017-06-06;25(6):1348-1361.e8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  16. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth
    Authors: JC Klein, K Moses, G Zelinskyy, S Sody, J Buer, S Lang, I Helfrich, U Dittmer, CJ Kirschning, S Brandau
    Nat Commun, 2017-03-16;8(0):14600.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Activation of Gpr109a, receptor for niacin and the commensal metabolite butyrate, suppresses colonic inflammation and carcinogenesis.
    Authors: Singh N, Gurav A, Sivaprakasam S, Brady E, Padia R, Shi H, Thangaraju M, Prasad P, Manicassamy S, Munn D, Lee J, Offermanns S, Ganapathy V
    Immunity, 2014-01-09;40(1):128-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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