Mouse L1CAM PE-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: L1CAM

L1CAM, also known as Neural Cell Adhesion Molecule L1 (NCAM-L1) and CD171, is a 200‑220 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, L1/Neurofascin/NgCAM family of molecules. L1CAM is expressed by neurons, especially by growing axons on their growth cones. Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express L1CAM. Mature mouse L1CAM consists of a 1104 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains and 5 fibronectin type-III domains, a 23 aa transmembrane region, and a 114 aa cytoplasmic tail. Mouse L1CAM shares 88% aa sequence identity with human L1CAM. L1CAM is critical for neural development. Specifically, L1CAM plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination. L1CAM mediates homophilic cell-cell interaction but also binds heterophilically with Axonin-1, CD24, CD9, Neurocan and several Integrins. L1CAM can undergo membrane‑proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble ECD and the generation of a membrane‑bound stub. The soluble ECD can serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration. L1CAM also plays a role in the ontogeny of human tumors, and its expression is linked to poor prognosis. Proteolytic processing by ADAM10 and presenilin/ gamma -secretase is essential for "forward signaling" where an L1CAM intracellular domain translocates to the nucleus and participates in gene regulation. Defects in L1CAM are the cause of the neurological MASA/CRASH syndrome. In addition, uncleaved L1CAM can cluster with Integrin alpha 5 either in-cis, or in-trans, inducing IL-1 beta expression and subsequent NF- kappa B activation. This is referred to as "reverse signaling".

Product Datasheets

Related Research Areas

• Adhesion Molecules in Angiogenesis
• B Cell Chemotaxis, Migration, and Adhesion
• Cancer Biomarkers
• Cancer Stem Cell Markers
• Dendritic Cell Adhesion and Migration
• Epithelial Cell Markers and Intracellular Molecules
• Immunoglobulin Superfamily CAMs
• Monocyte Markers
• Neuronal Lineage Markers
• Semaphorins, Plexin Receptors, and Related Molecules
• Surface Ectodermal Cells
• Synaptic Adhesion Molecules
• Synaptic Proteins
• T Cell Migration/Adhesion