Mouse Laminin-1 Antibody

Catalog # Availability Size / Price Qty
MAB25491
MAB25491-SP
Laminin‑1 in Mouse Embryo.
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Mouse Laminin-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Laminin‑1 in Western blots. This antibody reacts with mouse Laminin-1 beta chain (1).
Source
Monoclonal Rat IgG1 Clone # AL-3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot

Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. This application was not tested by R&D Systems.

 
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Laminin-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Laminin‑1 in Mouse Embryo. Laminin-1 was detected in immersion fixed frozen sections of embryonic mouse parietal endoderm (E10.5) using Rat Anti-Mouse Laminin-1 Monoclonal Antibody (Catalog # MAB25491) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin-1

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival (1, 2).

References
  1. Skubitz, A.P. et al. (1988) J Biol Chem. 263:4861
  2. Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.
Alternate Names
LAMA; LAMA1; laminin subunit alpha 1; Laminin1; Laminin-1; Laminin-111; PTBHS; S-LAM-alpha

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Reviews for Mouse Laminin-1 Antibody

Average Rating: 5 (Based on 2 Reviews)

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Mouse Laminin-1 Antibody
By Anonymous on 07/21/2021
Application: IHC Sample Tested: Kidney tissue Species: Mouse

Kidney sections were stained with anti-Laminin antibody (red) to serve as a marker for the basement membrane.


Mouse Laminin-1 Antibody
By Anonymous on 07/02/2021
Application: IHC Sample Tested: Lung tissue Species: Mouse