Mouse Laminin  alpha 1 / beta 1 Antibody

Catalog # Availability Size / Price Qty
MAB4656
MAB4656-SP
Laminin  alpha 1/ beta 1 in Embryonic Mouse Decidua.
2 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)

Mouse Laminin  alpha 1 / beta 1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects the Hep-1 heparin binding domain of mouse Laminin A/alpha 1 subunit (1).
Source
Monoclonal Rat IgG2A Clone # AL-4
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Purified fragment of chymotrypsin-digested mouse EHS tumor-derived Laminin-1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot

Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349. This application was not tested by R&D Systems.

 
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Laminin a1 antibody in Embryonic Mouse Decidua by Immunohistochemistry (IHC-Fr). View Larger

Laminin alpha 1/ beta 1 in Embryonic Mouse Decidua. Laminin alpha 1/ beta 1 was detected in immersion fixed frozen sections of embryonic mouse decidua (E10.5) using 10 µg/mL Rat Anti-Mouse Laminin alpha 1/ beta 1 Monoclonal Antibody (Catalog # MAB4656) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Laminin a1 antibody in Mouse Kidney, Skin, Intestine, and Skeletal Muscle by Immunohistochemistry (IHC-Fr). View Larger

Laminin alpha 1/ beta 1 in Mouse Kidney, Skin, Intestine, and Skeletal Muscle. Laminin alpha 1/ beta 1 was detected in perfusion fixed frozen sections of mouse kidney glomeruli (panel A), skin (panel B), intestine (panel C), and skeletal muscle (panel D) using Rat Anti-Mouse Laminin alpha 1/ beta 1 Monoclonal Antibody (Catalog # MAB4656) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 1

Laminins are heterotrimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan, and other receptors, Laminins contribute to cell differentation, cell shape and migration, maintenance of tissue phenotypes, and survival. Laminin-1 is comprised of alpha 1, beta 1, and gamma 1 subunits.

References
  1. Skubitz, A.P. et al. (1988) J. Biol. Chem. 263:4861.
  2. Skubitz, A.P. et al. (1987) Exp. Cell Res. 173:349.
Entrez Gene IDs
284217 (Human); 16772 (Mouse); 316758 (Rat)
Alternate Names
LAMA; LAMA1 laminin, alpha 1 ; LAMA1; Laminin alpha 1; S-LAM-alpha

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Citations for Mouse Laminin  alpha 1 / beta 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Identification of tyrosine brominated extracellular matrix proteins in normal and fibrotic lung tissues
    Authors: Cruz, LC;Habibovic, A;Dempsey, B;Massafera, MP;Janssen-Heininger, YMW;Lin, MJ;Hoffman, ET;Weiss, DJ;Huang, SK;van der Vliet, A;Meotti, FC;
    Redox biology
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Identification of the niche and mobilization mechanism for tissue-protective multipotential bone marrow ILC progenitors
    Authors: Q Liu, JH Lee, HM Kang, CH Kim
    Science Advances, 2022-11-23;8(47):eabq1551.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Increased Expression of Laminin Subunit Alpha 1 Chain by dCas9-VP160
    Authors: A Perrin, J Rousseau, JP Tremblay
    Mol Ther Nucleic Acids, 2016-12-10;6(0):68-79.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Western Blot

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Mouse Laminin  alpha 1 Antibody
By Anonymous on 09/18/2019
Application: ICC/IF Sample Tested: Adult brain Species: Mouse

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