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Mouse Laminin  alpha 4 Antibody

Catalog #: AF3837
16 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3837
AF3837-SP
Laminin alpha 4 in Mouse Embryo.
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Product Details
Citations (16)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Laminin  alpha 4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Laminin  alpha 4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Laminin alpha 4
Gln826-Ala1816
Accession # P97927
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Laminin  alpha 4
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Laminin a4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Laminin alpha 4 in Mouse Embryo. Laminin a4 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Laminin a4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3837) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to membranes in blood vessels in CNS. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Laminin alpha 4

Laminins are heterotrimeric glycoproteins and are major components of the basement membrane. Each laminin is comprised of a single alpha, beta, and gamma chain that heterotrimerize via their coiled-coil domains to form a large cruciform-shaped molecule. Mature Laminin  alpha 4 is a subunit of Laminin 411, Laminin 421, and Laminin 423. Mature Laminin alpha 4 is a 1792 amino acid (aa) residue protein that has a Laminin N-terminal domain, four Laminin EGF-like domains, the coiled-coil domain, and five C‑terminal tandem Laminin G-like domains. The G-like domains contain binding sites for integrin, heparin, and dystroglycan. Within the region used as immunogen, mouse Laminin  alpha 4 shows 91% and 97% aa sequence identity with human and rat Laminin  alpha 4, respectively.

Entrez Gene IDs
3910 (Human); 16775 (Mouse); 309816 (Rat)
Alternate Names
DKFZp686D23145; LAMA3LAMA4*-1; LAMA4; laminin alpha 4 chain; Laminin alpha 4; laminin subunit alpha-4; laminin, alpha 4; Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha

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Citations for Mouse Laminin  alpha 4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Micro-laminin gene therapy can function as an inhibitor of muscle disease in the dy(W) mouse model of MDC1A
    Authors: Packer D, Martin PT.
    Mol Ther Methods Clin Dev
  2. Deficiency of Stabilin-1 in the Context of Hepatic Melanoma Metastasis
    Authors: Wohlfeil, SA;Olsavszky, A;Irkens, AL;Häfele, V;Dietsch, B;Straub, N;Goerdt, S;Géraud, C;
    Cancers
    Species: Transgenic Mouse, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Transcriptomics and translatomics identify a robust inflammatory gene signature in brain endothelial cells after ischemic stroke
    Authors: Arbaizar-Rovirosa, M;Gallizioli, M;Lozano, JJ;Sidorova, J;Pedragosa, J;Figuerola, S;Chaparro-Cabanillas, N;Boya, P;Graupera, M;Claret, M;Urra, X;Planas, AM;
    Journal of neuroinflammation
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Single-cell dissection of the obesity-exercise axis in adipose-muscle tissues implies a critical role for mesenchymal stem cells
    Authors: Jiekun Yang, Maria Vamvini, Pasquale Nigro, Li-Lun Ho, Kyriakitsa Galani, Marcus Alvarez et al.
    Cell Metabolism
  5. Astrocyte-targeted gene delivery of interleukin 2 specifically increases brain-resident regulatory T cell numbers and protects against pathological neuroinflammation
    Authors: Lidia Yshii, Emanuela Pasciuto, Pascal Bielefeld, Loriana Mascali, Pierre Lemaitre, Marika Marino et al.
    Nature Immunology
  6. Pericyte-to-endothelial cell signaling via vitronectin-integrin regulates blood-CNS barrier
    Authors: Swathi Ayloo, Christopher Gallego Lazo, Shenghuan Sun, Wei Zhang, Bianxiao Cui, Chenghua Gu
    Neuron
  7. Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment
    Authors: SA Wohlfeil, V Häfele, B Dietsch, C Weller, C Sticht, AS Jauch, M Winkler, CD Schmid, AL Irkens, A Olsavszky, K Schledzews, PS Reiners-Ko, S Goerdt, C Géraud
    Journal of Translational Medicine, 2022-02-02;20(1):62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF
  8. Automated Quantitative Analysis of ex vivo Blood-Brain Barrier Permeability Using Intellesis Machine-Learning
    Authors: M Nesbit, JC Mamo, M Majimbi, V Lam, R Takechi
    Frontiers in Neuroscience, 2021-04-16;15(0):617221.
    Species: Mouse, Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition
    Authors: Emanuela Pasciuto, Oliver T. Burton, Carlos P. Roca, Vasiliki Lagou, Wenson D. Rajan, Tom Theys et al.
    Cell
  10. The Adult Murine Intestine is Dependent on Constitutive Laminin-gamma1 Synthesis
    Authors: B Fields, A DeLaForest, M Zogg, J May, C Hagen, K Komnick, J Wieser, A Lundberg, H Weiler, MA Battle, KS Carlson
    Sci Rep, 2019-12-17;9(1):19303.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Immature monocytes recruited to the ischemic mouse brain differentiate into macrophages with features of alternative activation
    Authors: Francesc Miró-Mur, Isabel Pérez-de-Puig, Maura Ferrer-Ferrer, Xabier Urra, Carles Justicia, Angel Chamorro et al.
    Brain, Behavior, and Immunity
  12. Prenatal muscle development in a mouse model for the secondary dystroglycanopathies.
    Authors: Kim J, Hopkinson M, Kavishwar M, Fernandez-Fuente M, Brown S
    Skelet Muscle, 2016-02-19;6(0):3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Astrocytic laminin regulates pericyte differentiation and maintains blood brain barrier integrity.
    Authors: Yao Y, Chen Z, Norris E, Strickland S
    Nat Commun, 2014-03-03;5(0):3413.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Transforming growth factor β-1 stimulates profibrotic epithelial signaling to activate pericyte-myofibroblast transition in obstructive kidney fibrosis.
    Am J Pathol, 2012-11-09;182(1):118-31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Laminin-411 Is a Vascular Ligand for MCAM and Facilitates TH17 Cell Entry into the CNS.
    Authors: Flanagan K, Fitzgerald K, Baker J
    PLoS ONE, 2012-07-06;7(7):e40443.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  16. Fukutin-related protein alters the deposition of laminin in the eye and brain.
    Authors: Ackroyd MR, Whitmore C, Prior S, Kaluarachchi M, Nikolic M, Mayer U, Muntoni F, Brown SC
    J. Neurosci., 2011-09-07;31(36):12927-35.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Mouse Laminin  alpha 4 Antibody
By Anonymous on 07/16/2019
Application: IHC Sample Tested: Brain tissue Species: Mouse

Dilution 1:400

Immunohistochemistry Mouse Laminin  alpha 4 Antibody AF3837