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Mouse Leptin R Antibody

Catalog #: AF497
22 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF497
AF497-SP
Leptin R in Rat Brain.
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Product Details
Citations (22)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Leptin R Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Leptin R in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Leptin R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Leptin R
Ala20-Gly839
Accession # Q3US58
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR)
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Leptin R antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

Leptin R in Rat Brain. Leptin R was detected in perfusion fixed frozen sections of rat brain using 15 µg/mL Goat Anti-Mouse Leptin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF497) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the choroid plexus. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin R

Leptin receptor (OB-R), also named B219, is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and the LIF receptor. Multiple isoforms of human and mouse OB-R, including a long form (OB-RL) with a large cytoplasmic domain capable of signal-transduction, and several receptor isoforms with short cytoplasmic domains (OB-Rs) lacking signal-transducing capabilities, have been identified. The extracellular domains of the short and long forms of OB-R are identical. An OB-R transcript lacking a transmembrane domain and potentially encoding a soluble form of the receptor has also been described. Circulating soluble OB-R, complexed to leptin, has been detected in mouse serum. Serum soluble OB-R levels have been shown to increase during pregnancy. OB-RL transcripts were reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. Expression of OB-Rs transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney and primitive hematopoietic cell populations. OB-R has recently been shown to be encoded by the mouse diabetes (db) and rat fatty (fa) genes. Rodents homozygous for the db or fa mutations have been known to exhibit an obesity phenotype.

Mouse OB-R long form encodes a 1162 amino acid (aa) residue precursor protein with a 22 aa residue signal peptide, an 817 aa residue extracellular domain, a 21 aa residue transmembrane domain, and a 302 aa residue cytoplasmic domain. The extracellular domain of OB-R contains two hemopoietin receptor domains, a fibronectin type III domain and the WSXWS domain. Recombinant murine soluble OB-R has been shown to bind leptin with high affinity and is a potent leptin antagonist.

References
  1. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  2. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
  3. Lee, J.I. and J.M. Friedman (1996) Nature 379:632.
  4. Tartaglia, L.A. (1997) J. Biol. Chem. 272:6093.
  5. Gavrilova, O. et al. (1997) J. Biol. Chem. 272:30546.
Long Name
Leptin Receptor
Entrez Gene IDs
3953 (Human); 16847 (Mouse)
Alternate Names
B219; CD295 antigen; CD295; DB; DKFZp686B1731; huB219; LEPR; LEP-R; Leptin R; leptin receptor; LeptinR; OB R; OB receptor; OB-R; OBRCD295

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Citations for Mouse Leptin R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Bone metastasis initiation is coupled with bone remodeling through osteogenic differentiation of NG2+ cells
    Authors: W Zhang, Z Xu, X Hao, T He, J Li, Y Shen, K Liu, Y Gao, J Liu, D Edwards, AM Muscarella, L Wu, L Yu, L Xu, X Chen, YH Wu, IL Bado, Y Ding, S Aguirre, H Wang, Z Gugala, RL Satcher, ST Wong, XH Zhang
    Cancer Discovery, 2023-02-06;0(0):.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Ras drives malignancy through stem cell crosstalk with the microenvironment
    Authors: S Yuan, KS Stewart, Y Yang, MD Abdusselam, SM Parigi, TY Feinberg, K Tumaneng, H Yang, JM Levorse, L Polak, D Ng, E Fuchs
    Nature, 2022-11-30;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
    Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
    Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  4. Primary cilia mediate early life programming of adiposity through lysosomal regulation in the developing mouse hypothalamus
    Authors: CH Lee, DK Song, CB Park, J Choi, GM Kang, SH Shin, I Kwon, S Park, S Kim, JY Kim, H Dugu, JW Park, JH Choi, SH Min, JW Sohn, MS Kim
    Nat Commun, 2020-11-13;11(1):5772.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Chronic myelogenous leukemia stem cells require cell-autonomous pleiotrophin signaling
    Authors: HA Himburg, M Roos, T Fang, Y Zhang, CM Termini, L Schlussel, MM Kim, A Pang, J Kan, L Zhao, H Suh, JP Sasine, G Sapparapu, PM Bowers, G Schiller, JP Chute
    J. Clin. Invest., 2020-01-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Haematopoietic stem cells in perisinusoidal niches are protected from ageing
    Authors: M Saçma, J Pospiech, R Bogeska, W de Back, JP Mallm, V Sakk, K Soller, G Marka, A Vollmer, R Karns, N Cabezas-Wa, A Trumpp, S Méndez-Fer, MD Milsom, MA Mulaw, H Geiger, MC Florian
    Nat. Cell Biol., 2019-11-04;21(11):1309-1320.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. Stress-Induced Changes in Bone Marrow Stromal Cell Populations Revealed through Single-Cell Protein Expression Mapping
    Authors: N Severe, NM Karabacak, K Gustafsson, N Baryawno, G Courties, Y Kfoury, KD Kokkaliari, C Rhee, D Lee, EW Scadden, JE Garcia-Rob, T Brouse, M Nahrendorf, M Toner, DT Scadden
    Cell Stem Cell, 2019-07-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. A spontaneous leptin receptor point mutation causes obesity and differentially affects leptin signaling in hypothalamic nuclei resulting in metabolic dysfunctions distinct from db/db mice
    Authors: F Piattini, C Le Foll, J Kisielow, E Rosenwald, P Nielsen, T Lutz, C Schneider, M Kopf
    Mol Metab, 2019-04-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Aged marrow macrophages expand platelet-biased hematopoietic stem cells via Interleukin1B
    Authors: BJ Frisch, CM Hoffman, SE Latchney, MW LaMere, J Myers, J Ashton, AJ Li, J Saunders, J Palis, AS Perkins, A McCabe, JN Smith, KE McGrath, F Rivera-Esc, A McDavid, JL Liesveld, VA Korshunov, MR Elliott, KC MacNamara, MW Becker, LM Calvi
    JCI Insight, 2019-04-18;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Involvement of leptin signaling in the development of cannabinoid CB2 receptor-dependent mirror image pain
    Authors: C Nozaki, E Nent, A Bilkei-Gor, A Zimmer
    Sci Rep, 2018-07-17;8(1):10827.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Aberrant TGF-? activation in bone tendon insertion induces enthesopathy-like disease
    Authors: X Wang, L Xie, J Crane, G Zhen, F Li, P Yang, M Gao, R Deng, Y Wang, X Jia, C Fan, M Wan, X Cao
    J. Clin. Invest., 2018-01-22;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2017-11-13;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Programmed cell senescence in skeleton during late puberty
    Authors: C Li, Y Chai, L Wang, B Gao, H Chen, P Gao, FQ Zhou, X Luo, JL Crane, B Yu, X Cao, M Wan
    Nat Commun, 2017-11-03;8(1):1312.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  14. Data on the expression of leptin and leptin receptor in the dorsal root ganglion and spinal cord after preganglionic cervical root avulsion
    Authors: KT Chang, YL Lin, CT Lin, MJ Tsai, WC Huang, YH Shih, YY Lee, H Cheng, MC Huang
    Data Brief, 2017-10-07;15(0):567-572.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. A dangerous liaison: Leptin and sPLA2-IIA join forces to induce proliferation and migration of astrocytoma cells
    Authors: R Martín, C Cordova, B Gutiérrez, M Hernández, ML Nieto
    PLoS ONE, 2017-03-01;12(3):e0170675.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Differential cytokine contributions of perivascular haematopoietic stem cell niches
    Authors: N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette
    Nat. Cell Biol, 2017-02-20;19(3):214-223.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Hypomorphism for RPGRIP1L, a ciliary gene vicinal to the FTO locus, causes increased adiposity in mice.
    Authors: Stratigopoulos G, Martin Carli J, O'Day D, Wang L, Leduc C, Lanzano P, Chung W, Rosenbaum M, Egli D, Doherty D, Leibel R
    Cell Metab, 2014-05-06;19(5):767-79.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC, Immunoprecipitation
  18. Kinetics of leptin binding to the Q223R leptin receptor.
    Authors: Verkerke H, Naylor C, Zabeau L, Tavernier J, Petri W, Marie C
    PLoS ONE, 2014-04-17;9(4):e94843.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  19. Deletion of selenoprotein M leads to obesity without cognitive deficits.
    Authors: Pitts, Matthew, Reeves, Mariclai, Hashimoto, Ann C, Ogawa, Ashley, Kremer, Penny, Seale, Lucia A, Berry, Marla J
    J Biol Chem, 2013-07-23;288(36):26121-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Leptin enhances CC-chemokine ligand expression in cultured murine macrophage.
    Authors: Kiguchi N, Maeda T, Kobayashi Y, Fukazawa Y, Kishioka S
    Biochem. Biophys. Res. Commun., 2009-05-03;384(3):311-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. Soluble receptor inhibits leptin transport.
    Authors: Tu H, Kastin AJ, Hsuchou H, Pan W
    J. Cell. Physiol., 2008-02-01;214(2):301-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  22. High circulating leptin receptors with normal leptin sensitivity in liver-specific insulin receptor knock-out (LIRKO) mice.
    Authors: Cohen SE, Kokkotou E, Biddinger SB, Kondo T, Gebhardt R, Kratzsch J, Mantzoros CS, Kahn CR
    J. Biol. Chem., 2007-06-07;282(32):23672-8.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot

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Reviews for Mouse Leptin R Antibody

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Mouse Leptin R Antibody
By Anonymous on 08/31/2018
Application: ICC/IF Sample Tested: bone marrow Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse Leptin R Antibody AF497
Mouse Leptin R Antibody
By Michele Doucet on 04/21/2016
Application: Flow Sample Tested: Bone marrow cells Species: Goat

0.2ug/ml/10^6 cells