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Mouse Limitin/IFN-zeta Antibody

Catalog #: AF597
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF597
AF597-SP
Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse Limitin Antibody.
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Mouse Limitin/IFN-zeta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects Limitin/IFN‑ zeta in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IFN-gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Limitin/IFN‑ zeta
Leu22-Arg182
Accession # Q9R1T0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.01 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Limitin/IFN‑ zeta (Catalog # 1535-LM)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse thymus
Neutralization
Measured by its ability to neutralize Limitin inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 1.5-7.5 ng/mL in the presence of 30 pg/mL Recombinant Mouse Limitin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse Limitin Antibody. View Larger

Limitin Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse Limitin Antibody. Recombinant Mouse Limitin (Catalog # 1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Limitin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF597). The ND50 is typically 1.5-7.5 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Limitin/IFN-zeta

Limitin, also known as IFN-zeta, is a 20 kDa glycoprotein that is secreted by T cells and bronchial and salivary duct epithelial cells. It inhibits B-lineage cell development but has little effect on myelopoiesis or erythropoiesis. Limitin binds to IFN-alpha / beta receptors and has potent antiviral activity. Mouse Limitin shares 32%, 26%, and 30% amino acid sequence identity with mouse IFN-alpha, mouse IFN-beta, and human IFN-omega, respectively.

Long Name
Interferon zeta
Entrez Gene IDs
319146 (Mouse)
Alternate Names
6030405N23Rik; BGIF; Ifnz; IFN-z; IFNzeta; interferon zeta; Limitin; Lmtn

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Citation for Mouse Limitin/IFN-zeta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Relevance of MIC-1 in the Era of PSA as a Serum Based Predictor of Prostate Cancer: A Critical Evaluation
    Authors: N Bansal, D Kumar, A Gupta, D Chandra, SN Sankhwar, A Mandhani
    Sci Rep, 2017-12-04;7(1):16824.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot

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