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Mouse LIMPII/SR-B2 Lumenal Loop Antibody

Catalog #: AF1888
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1888
AF1888-SP
Detection of LIMPII/SR-B2 in Mouse Splenocytes by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse LIMPII/SR-B2 Lumenal Loop Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LIMPII/SR‑B2 Lumenal Loop in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human (rh) LIMPII is observed and less than 1% cross‑reactivity with rhCD36 and rhSR‑B1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LIMPII/SR‑B2 Lumenal Loop
Arg27-Thr432
Accession # O35114
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse LIMPII/SR-B2 Fc Chimera (Catalog # 1888-LM)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of LIMPII/SR-B2 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of LIMPII/SR-B2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse LIMPII/SR-B2 Affinity-purified Polyclonal Antibody (Catalog # AF1888, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIMPII/SR-B2

LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1‑3). SR-B/CD36 family members are type III integral membrane proteins with an N‑ as well as a C-terminal cytoplasmic tail, and a large extracellular (or luminal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is expressed on all tissues and cell types examined so far, including activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII‑thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation and inflammation (1). Overexpression of LIMPII causes an enlargement of early and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).

References
  1. Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855. 
  2. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. 
  3. Eskelinen, E-L. et al. (2003) Trends in Cell Biol. 13:137.
  4. Kuronita, T. et al. (2002) J. Cell Sci. 115:4117.
  5. Gamp, A-C. et al. (2003) Human Mol. Genet. 12:631.
Long Name
Lysosomal Integral Membrane Protein II
Entrez Gene IDs
950 (Human); 12492 (Mouse)
Alternate Names
AMRF; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II); CD36 antigen; CD36L2; CD36L2LIMP II; HLGP85; LGP85; LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2; LIMPII; LIMPIICD36 antigen-like 2; LPG85; lysosome membrane protein 2; Lysosome membrane protein II; SCARB2; Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein; scavenger receptor class B, member 2; SRB2; SR-B2; SR-BII

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Citations for Mouse LIMPII/SR-B2 Lumenal Loop Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Lung immunoglobulin A immunity dysregulation in cystic fibrosis
    Authors: AM Collin, M Lecocq, S Noel, B Detry, FM Carlier, F Aboubakar, C Bouzin, T Leal, M Vermeersch, V De Rose, L Regard, C Martin, PR Burgel, D Hoton, S Verleden, A Froidure, C Pilette, S Gohy
    EBioMedicine, 2020-09-11;60(0):102974.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Detection
  2. HSL-knockout mouse testis exhibits class B scavenger receptor upregulation and disrupted lipid raft microdomains.
    Authors: Casado M, Huerta L, Ortiz A, Perez-Crespo M, Gutierrez-Adan A, Kraemer F, Lasuncion M, Busto R, Martin-Hidalgo A
    J Lipid Res, 2012-09-17;53(12):2586-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Identification of a Human SCARB2 Region That Is Important for Enterovirus 71 Binding and Infection.
    Authors: Yamayoshi S, Koike S
    J. Virol., 2011-03-09;85(10):4937-46.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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