Mouse Mast Cell Protease-11/Prss34 Antibody

Catalog #: MAB2857 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2857
MAB2857-SP

All Mast Cell Protease-11/Prss34 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Mast Cell Protease-11/Prss34 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Mast Cell Protease‑11/Prss34 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) MCP-1, rmMCP-6, or rmMCP-7 is observed.

Source

Monoclonal Rat IgG₁ Clone # 451421

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑11/Prss34
Met20-Ser318
Accession # Q80UR4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Mast Cell Protease‑11/Prss34 (Catalog # 2857-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease‑11/Prss34 (Catalog # 2857-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mast Cell Protease-11/Prss34

Mast Cell Protease-11 (MCP-11) is encoded by Prss34, one of 13 genes on mouse chromosome 17A3.3 that correspond to functional trypsin-like serine proteases (1). The deduced amino acid sequence of mouse MCP-11 consists of 318 residues with a signal peptide (residues 1 to 19), a pro region (residue 20 to 34), and a catalytic domain (35 to 318). The mRNA is preferentially expressed in spleen and bone marrow. The amino acid sequence of mouse MCP-11 shares 84% and 60% identity with that of rat and dog, respectively. The human gene corresponding to Prss34 contains a mutation that leads to a premature translation termination codon, thus encoding a protein that is unlikely to be enzymatically active.

References

Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.

Entrez Gene IDs

328780 (Mouse)

Alternate Names

EOS; Mast Cell Protease11; Mast Cell Protease-11; MCP-11; MCPT11; Prss34

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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