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Mouse Mast Cell Protease-6/Mcpt6 Antibody

Catalog #: AF3736
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3736
AF3736-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Mast Cell Protease-6/Mcpt6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects Mast Cell Protease-6/Mcpt6 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) TPS-1 and rhTPS-B2 is observed and less then 10% cross-reactivity with recombinant mouse MCP-7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease-6/Mcpt6 Ala22-Ser276 Accession # P21845
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (Catalog # 3736-SE)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (Catalog # 3736-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (0.5 µg/mL, Catalog # 3736‑SE) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002 ). The Neutralization Dose (ND50) is typically 3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: Mast Cell Protease-6/Mcpt6

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast cell protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, MCP-6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, MCP-6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). MCP-6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.

References
  1. Caughey, G. H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1535 .
  2. Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
  3. Hallgren, J. and G. Pejler, (2006) FEBS J. 273:1871.
Entrez Gene IDs
7177 (Human); 17229 (Mouse); 29268 (Rat)
Alternate Names
Mast Cell Protease6; Mast Cell Protease-6; Mcpt6

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Citation for Mouse Mast Cell Protease-6/Mcpt6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Nerve growth factor partially recovers inflamed skin from stress-induced worsening in allergic inflammation.
    Authors: Peters EM, Liezmann C, Spatz K, Daniltchenko M, Joachim R, Gimenez-Rivera A, Hendrix S, Botchkarev VA, Brandner JM, Klapp BF
    J. Invest. Dermatol., 2010-11-18;131(3):735-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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