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Mouse MGL1/2 (CD301a/b) Antibody

Catalog #: AF4297
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4297
AF4297-SP
Detection of MGL1/2 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MGL1/2 (CD301a/b) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MGL1 and MGL2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MGL1 Gln57-Ser304 Accession # AAH14811
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse MGL1/2
Flow Cytometry
2.5 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.4-1.6 µg/mL of this antibody will block 50% of the binding of 500 ng/mL of biotinylated Lex-Polyacrylamide to immobilized Recombinant Mouse MGL1 (Catalog # 4297-MG) coated at 2.5 µg/mL (100 µL/well). At 15 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MGL1/2 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of MGL1/2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse MGL1/2 (CD301a/b) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4297, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: MGL1/2 (CD301a/b)

Mouse MGL1 (macrophage galactose N-acetyl-galactosamine (GalNAc) specific Lectin 1, CD301a), also called ASGP-BP (asialoglycoprotein binding protein), is a 38 kDa type II transmembrane glycoprotein of the C-type lectin family (1). Two MGL proteins are encoded by separate genes in the mouse, but share 91% amino acid (aa) identity in the extracellular domain (ECD) (2). Only one MGL occurs in human and rat, and this is more structurally similar to mouse MGL1 than MGL2. However, mouse MGL1 binds Lewis X, in contrast to human MGL and mouse MGL2 which both bind specifically to terminal GalNAc residues (2). Lewis X is a trisaccharide commonly found on leukocytes and some tumor cells. Both mouse MGL proteins are expressed on immature dendritic cells. Mouse MGL1 and MGL2 are markers for connective tissue macrophages of a type termed alternately activated macrophages. These macrophages are induced by IL-4 that is produced during Th2-mediated inflammatory responses to parasitic infections or allergic airway inflammation (3, 4). Quantitative RT-PCR after helminth infection shows a peak of MGL1 expression at 7 days, while MGL2 shows increasing expression for at least 29 days (3). This, and data from MGL1 knockout mice (5), indicates that MGL1 is critical during the formation of granulation tissue, with MGL2 remaining involved during chronic infection. Mouse MGL1 is synthesized with an N-terminal 35 aa cytoplasmic region, a 21 aa transmembrane segment and a 248 aa ECD. The ECD contains one 129 aa carbohydrate recognition domain (CRD) that shows 78% and 63% aa identity with rat and human MGL, respectively.

References
  1. Sato, M. et al. (1992) J. Biochem. 111:331.
  2. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
  3. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
  4. Sato, K. et al. (2005) Int. Immunol. 17:559.
  5. Sato, K. et al. (2005) Blood 106:207.
Long Name
Macrophage Galactose-type C-lectin 1 and 2
Alternate Names
CD8; Leu2; MGL1/2; p32

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Citations for Mouse MGL1/2 (CD301a/b) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Kupffer cell receptor CLEC4F is important for the destruction of desialylated platelets in mice
    Authors: Y Jiang, Y Tang, C Hoover, Y Kondo, D Huang, D Restagno, B Shao, L Gao, J Michael Mc, M Zhou, R Silasi-Man, S McGee, M Jiang, X Bai, F Lupu, C Ruan, JD Marth, D Wu, Y Han, L Xia
    Cell Death and Differentiation, 2021-05-15;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice
    Authors: J Chida, H Hara, K Uchiyama, E Takahashi, H Miyata, H Kosako, Y Tomioka, T Ito, H Horiuchi, H Matsuda, H Kido, S Sakaguchi
    PLoS Pathog., 2020-08-26;16(8):e1008823.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC, Western Blot
  3. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  4. IkappaB kinase activity drives fetal lung macrophage maturation along a non-M1/M2 paradigm.
    Authors: Stouch A, Zaynagetdinov R, Barham W, Stinnett A, Slaughter J, Yull F, Hoffman H, Blackwell T, Prince L
    J Immunol, 2014-06-30;193(3):1184-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. T-cell recruitment and Th1 polarization in adipose tissue during diet-induced obesity in C57BL/6 mice.
    Authors: Strissel KJ, DeFuria J, Shaul ME, Bennett G, Greenberg AS, Obin MS
    Obesity (Silver Spring), 2010-01-28;18(10):1918-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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