Mouse NCAM-1/CD56 PE-conjugated Antibody

Catalog #: FAB7820P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB7820P-100UG

Detection of NCAM‑1/CD56 in Neuro‑2A Mouse Cell Line by Flow Cytometry.

1 Image

All NCAM-1/CD56 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NCAM-1/CD56 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse NCAM‑1/CD56 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 809220

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse NCAM‑1/CD56
Leu20-Thr711
Accession # P13595

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of NCAM‑1/CD56 in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro-2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB7820P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NCAM-1/CD56

NCAM-1 (Neural adhesion molecule-1; also CD56) is a 120-190 kDa glycoprotein member of the Ig Superfamily. It is expressed on multiple cell types, both in the embryo and adult. Here, it serves as both an adhesion molecule and a receptor for multiple ligands, including as FGFR, PDGF, GDNF and agrin. On the cell surface, it is a cis-oriented homodimer that can form homodimers in-trans with other cis-homodimers. In the embryo, NCAM-1 is polysialylated (PolySia), and shows a MW of 200-220 kDa in SDS-PAGE. This polysialylation reduces the ability of NCAM-1 to dimerize. Mature mouse NCAM-1 is a 1096 amino acid (aa) type I transmembrane (TM) protein (aa 20-1115). It possesses a 692 aa extracellular region (aa 20-711) and a 386 aa cytoplasmic domain. The extracellular region contains five consecutive C2-type Ig-like domains (aa 20-492) followed by two FN type-III domains (aa 497-692). Multiple splice variants exist. There is a 140 kDa TM variant that shows a deletion of aa 810-1076, and a 120 kDa variant that is GPI-linked and shows a 24 aa substitution for aa 702-1115. A third potential variant contains a five aa substitution for aa 601-1115. Over aa 20-711, mouse NCAM-1 shares 99% and 95% aa identity with rat and human NCAM-1, respectively.

Long Name

Neural Cell Adhesion Molecule

Entrez Gene IDs

4684 (Human); 17967 (Mouse); 24586 (Rat)

Alternate Names

CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM