Mouse Nectin-2/CD112 Antibody Summary
Gln32-Gly351 (predicted)
Accession # P32507
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Nectin-2/CD112 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Rat Anti-Mouse Nectin-2/CD112 Monoclonal Antibody (Catalog # MAB3869, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nectin-2/CD112
Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that control cell adhesion, proliferation, and migration (1, 2, 3). The name Nectin derives from the Latin word necto, which means “to connect”. The Nectin family contains four members (Nectin-1 to -4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma which is secreted), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been given the alternate name of poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Mouse Nectin-2 is a 70 to 78 kDa type I TM glycoprotein that is found on a variety of cell types (4, 5). It has two splice forms (4, 6, 7). Nectin-2 alpha /PRR2 is a 65 kDa short form and is synthesized as a 467 amino acid precursor. It contains a 31 aa signal sequence, a 315 aa extracellular domain (ECD), a 28 aa TM segment, and a 93 aa cytoplasmic region. The ECD contains one N-terminal V-type Ig domain and two 85‑95 aa C2-type Ig-like domains (aa 153‑337) (8). The V-domain is believed to mediate Nectin binding to its ligands (9). A long, 78 kDa, 530 aa isoform of mouse Nectin-2 (Nectin-2δ) also exists. It has the same signal sequence and extracellular domain as Nectin-2 alpha (aa 1‑338), but differs in the TM segment (21 aa in length) and cytoplasmic region (159 aa in length) (4, 6, 7). Mouse Nectin-2 ECD (aa 32 - 338) shares 72%, 77% and 95% aa identity with the ECD in human, canine and rat Nectin-2, respectively. Nectin-2 is known to bind pseudorabies virus, and herpes simplex virus-2 (HSV-2). It also binds select HSV-1 strains. It does not bind poliovirus (1, 10, 11). As a cell adhesion molecule, Nectin-2 will form cis‑homodimers (same cell) and trans-homodimers (across cells). Nectin-2 will not cis‑dimerize with other Nectins, but will trans-heterodimerize with Nectin-3 and CD266/DNAM-1 (1, 3, 11, 12, 13). Nectin-2 is found concentrated at cell-to-cell interfaces, and is presumed to contribute to tight and adherens junction formation (14). Through its interaction with NK and T cell expressed DNAM-1, it also promotes lymphocyte cytotoxicity and cytokine secretion against both tumors and dendritic cells (DC) expressing Nectin-2 (15, 16). In the case of DC, this may be a mechanism whereby the immune system eliminates DC that are inefficient at antigen presentation. Nectin-2 is expressed on epithelium, endothelial cells, Sertoli cells, monocytes, dendritic cells, granulosa cells, mast cells, eosinophils and fibroblasts.
- Takai, Y. and H. Nakanishi (2003) J. Cell Sci. 116:17.
- Rikitake, Y. and Y. Takai (2008) Cell. Mol. Life Sci. 65:253.
- Sasisaka, T. et al. (2007) Curr. Opin. Cell Biol. 19:1.
- Aoki, J. et al. (1994) J. Biol. Chem. 269:8431.
- Takahashi, K. et al. (1999) J. Cell Biol. 145:539.
- Aoki, J. et al. (1997) Exp. Cell Res. 235:374.
- Lopez, M. et al. (1998) Blood 92:4602.
- Morrison, M.E. and V.R. Racaniello (1992) J. Virol. 66:2807.
- Struyf, F. et al. (2002) J. Virol. 76:12940.
- Delboy, M.G. et al. (2006) Virology J. 3:105.
- Irie, K. et al. (2004) Semin. Cell Dev. Biol. 15:643.
- Tahara-Hanaoka, S. et al. (2004) Int. Immunol. 16:533.
- Satoh-Horikawa, K. et al. (2000) J. Biol. Chem. 275:10291.
- Nakanishi, H. and Y. Takai (2004) Biol. Chem. 385:885.
- Tahara-Hanaoka, S. et al. (2006) Blood 107:1491.
- Pende, D. et al. (2006) Blood 107:2030.
Product Datasheets
Citations for Mouse Nectin-2/CD112 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
6
Citations: Showing 1 - 6
Filter your results:
Filter by:
-
Single-cell RNA sequencing shows the immunosuppressive landscape and tumor heterogeneity of HBV-associated hepatocellular carcinoma
Authors: DW Ho, YM Tsui, LK Chan, KM Sze, X Zhang, JW Cheu, YT Chiu, JM Lee, AC Chan, ET Cheung, DT Yau, NH Chia, IL Lo, PC Sham, TT Cheung, CC Wong, IO Ng
Nature Communications, 2021-06-17;12(1):3684.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
CD112 Regulates Angiogenesis and T Cell Entry into the Spleen
Authors: E Russo, P Runge, NH Jahromi, H Naboth, A Landtwing, R Montecchi, N Leicht, MC Hunter, Y Takai, C Halin
Cells, 2021-01-15;10(1):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Natural killer cells impede the engraftment of cardiomyocytes derived from induced pluripotent stem cells in syngeneic mouse model
Authors: Y Nakamura, S Miyagawa, S Yoshida, S Sasawatari, T Toyofuku, K Toda, Y Sawa
Sci Rep, 2019-07-25;9(1):10840.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Laquinimod, a prototypic quinoline-3-carboxamide and aryl hydrocarbon receptor agonist, utilizes a CD155-mediated natural killer/dendritic cell interaction to suppress CNS autoimmunity
Authors: M Ott, E Avendaño-G, E Ullrich, C Dreyer, J Strauss, M Harden, M Schön, MP Schön, G Bernhardt, C Stadelmann, C Wegner, W Brück, S Nessler
J Neuroinflammation, 2019-02-26;16(1):49.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Blockade of the checkpoint receptor TIGIT prevents NK cell exhaustion and elicits potent anti-tumor immunity
Authors: Q Zhang, J Bi, X Zheng, Y Chen, H Wang, W Wu, Z Wang, Q Wu, H Peng, H Wei, R Sun, Z Tian
Nat. Immunol., 2018-06-18;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity.
Authors: Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N
Leukemia, 2013-11-13;28(6):1316-25.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse Nectin-2/CD112 Antibody
There are currently no reviews for this product. Be the first to review Mouse Nectin-2/CD112 Antibody and earn rewards!
Have you used Mouse Nectin-2/CD112 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
