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Mouse Neogenin Antibody

Catalog #: MAB1079
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1079
MAB1079-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Neogenin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Neogenin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse DCC is observed.
Source
Monoclonal Rat IgG2A Clone # 221519
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Neogenin
Ala42-Ile1033 (Asp442-Leu461 del)
Accession # NP_032710
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Neogenin Fc Chimera (Catalog # 1079-NE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neogenin

Neogenin (NEO) is a type I transmembrane protein that is crucial for axonal guidance and neuronal migration. It is also involved in regulating differentiation programs in many embryonic and adult tissues (1). Mouse NEO is widely expressed in adult tissues and is expressed throughout the mid to late stages of gestation, in both neuronal and non-neuronal tissues. It is a member of the immunoglobulin (Ig) superfamily and is closely related to deleted in colorectal cancer (DCC). Mouse NEO cDNA encodes a 1493 amino acid residue (aa) precursor with a putative 36 aa signal peptide, a 1100 aa extracellular domain with six Ig-like C2 type domains and three fibronectin type III domains, a 21 aa transmembrane domain, and a 345 aa cytoplasmic domain. At least five isoforms are produced in mice by alternative splicing. Mouse NEO shares 96%, 93%, and 86% aa sequence identity with rat, human, and chicken NEO, respectively. It also has 46% and 29% sequence homology with mouse DCC and C. elegans UNC40, a homolog of DCC. NEO and DCC, together with the UNC5 family of type I transmembrane proteins, are receptors for the netrin/UNC6 family of laminin-related bifunctional guidance molecules that both attract some axons and repel others (2, 3). In mouse, at least five netrins (netrin‑1, ‑3, -4, G1, and G2) have been identified (3-5). Mouse netrin-1 and netrin-3 have been shown to be ligands for mouse NEO.

References
  1. Keeling, S.L. et al. (1997) Oncogene 15:691.
  2. Hong, K. et al. (1999) Cell 97:927.
  3. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
  4. Nakashiba, T. et al. (2000) J. Neurosci. 20:6540.
  5. Nakashiba, T. et al. (2002) Mech. Dev. 111:47.
Entrez Gene IDs
4756 (Human); 18007 (Mouse)
Alternate Names
HsT17534; IGDCC2DKFZp547B146; Immunoglobulin superfamily DCC subclass member 2; NEO; NEO1; neogenin (chicken) homolog 1; neogenin 1; neogenin homolog 1; Neogenin; NGN; NGNDKFZp547A066

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Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.

Citation for Mouse Neogenin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. RGMa regulates cortical interneuron migration and differentiation.
    Authors: O'Leary, Conor, Cole, Stacey J, Langford, Michael, Hewage, Jayani, White, Amanda, Cooper, Helen M
    PLoS ONE, 2013-11-27;8(11):e81711.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. What is the light chain isotype of Mouse Neogenin Antibody, Catalog # MAB1079?

    • MAB1079 is kappa light chain.

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