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Mouse Netrin-1 Antibody

Catalog #: AF1109
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1109
AF1109-SP
Product Details
Citations (14)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Netrin-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Netrin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant chicken (rch) Netrin‑1 and recombinant human Netrin-1 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Netrin-G1a and rmNetrin-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Netrin-1
Val22-Ala603
Accession # AAC52971
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Netrin‑1 (Catalog # 1109-N1)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of Recombinant Mouse Netrin-1 (Catalog # 1109-N1) to immobilized Recombinant Rat UNC5H2 Fc Chimera (Catalog # 1006-UN) coated at 5 µg/mL (100 µL/well). At 40 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-1

Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for "one who guides"). The molecule's cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma -chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin-1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis, Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1-7).

References
  1. Puschel, A. (1999) Mech. Dev. 83:65.
  2. Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
  3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
  4. Madison, R. et al. (2000) Exp. Neurology 161:563.
  5. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  6. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
  7. Corset, V. et al. (2000) Nature 407:747.
Entrez Gene IDs
9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names
netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of

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Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

Citations for Mouse Netrin-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Netrin-1 co-cross-linked hydrogel accelerates diabetic wound healing in situ by modulating macrophage heterogeneity and promoting angiogenesis
    Authors: Shu, F;Huang, H;Xiao, S;Xia, Z;Zheng, Y;
    Bioactive materials
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. A TBX5-dependent compartment boundary patterns the cardiac interventricular septum
    Authors: Kathiriya, IS;Dominguez, MH;Rao, KS;Muncie-Vasic, JM;Patrick Devine, W;Hu, KM;Hota, SK;Garay, BI;Quintero, D;Goyal, P;Matthews, MN;Thomas, R;Sukonnik, T;Miguel-Perez, D;Winchester, S;Brower, EF;Forjaz, A;Wu, PH;Wirtz, D;Kiemen, AL;Bruneau, BG;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Netrin-1 promotes the immunosuppressive activity of MDSCs in colorectal cancer
    Authors: X Xia, Z Mao, W Wang, J Ma, J Tian, S Wang, K Yin
    Cancer Immunology Research, 2023-05-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Restoring bone marrow niche function rejuvenates aged hematopoietic stem cells by reactivating the DNA Damage Response
    Authors: P Ramalingam, MC Gutkin, MG Poulos, T Tillery, C Doughty, A Winiarski, AG Freire, S Rafii, D Redmond, JM Butler
    Nature Communications, 2023-04-10;14(1):2018.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  5. Allosteric potentiation of GABAA receptor single-channel conductance by netrin-1 during neuronal-excitation-induced inhibitory synaptic homeostasis
    Authors: ES Chan, Y Ge, YW So, YF Bai, L Liu, YT Wang
    Cell Reports, 2022-11-01;41(5):111584.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Endothelial Unc5B controls blood-brain barrier integrity
    Authors: K Boyé, LH Geraldo, J Furtado, L Pibouin-Fr, M Poulet, D Kim, B Nelson, Y Xu, L Jacob, N Maissa, D Agalliu, L Claesson-W, SL Ackerman, A Eichmann
    Nature Communications, 2022-03-04;13(1):1169.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. The expression of Netrin-1 in the MIA-induced osteoarthritic temporomandibular joint in mice
    Authors: M Xiao, Z Hu, H Jiang, C Li, H Guo, W Fang, X Long
    Scientific Reports, 2021-08-03;11(1):15695.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  8. Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells
    Authors: K Yamahara, T Nakagawa, J Ito, K Kinoshita, K Omori, N Yamamoto
    Neuropharmacology, 2017-04-01;119(0):26-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response
    Authors: T Lahlali, ML Plissonnie, C Romero-Lóp, M Michelet, B Ducarouge, A Berzal-Her, F Zoulim, P Mehlen, R Parent
    Cell Mol Gastroenterol Hepatol, 2016-01-09;2(3):281-301.e9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014-03-02;20(4):377-84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions.
    Authors: van Gils, Janine M, Ramkhelawon, Bhama, Fernandes, Luciana, Stewart, Merran C, Guo, Liang, Seibert, Tara, Menezes, Gustavo, Cara, Denise C, Chow, Camille, Kinane, T Bernar, Fisher, Edward A, Balcells, Mercedes, Alvarez-Leite, Jacqueli, Lacy-Hulbert, Adam, Moore, Kathryn
    Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  12. Netrin-1 promotes glioblastoma cell invasiveness and angiogenesis by multiple pathways including activation of RhoA, cathepsin B, and cAMP-response element-binding protein.
    Authors: Shimizu A, Nakayama H, Wang P, Konig C, Akino T, Sandlund J, Coma S, Italiano J, Mammoto A, Bielenberg D, Klagsbrun M
    J Biol Chem, 2012-11-29;288(4):2210-22.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Interfering with multimerization of netrin-1 receptors triggers tumor cell death.
    Authors: Mille F, Llambi F, Guix C, Delloye-Bourgeois C, Guenebeaud C, Castro-Obregon S, Bredesen DE, Thibert C, Mehlen P
    Cell Death Differ., 2009-06-19;16(10):1344-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Distinct roles of Rac1/Cdc42 and Rho/Rock for axon outgrowth and nucleokinesis of precerebellar neurons toward netrin 1.
    Authors: Causeret F, Hidalgo-Sanchez M, Fort P, Backer S, Popoff MR, Gauthier-Rouviere C, Bloch-Gallego E
    Development, 2004-05-19;131(12):2841-52.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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