Home / NKG2D/CD314 / Mouse NKG2D/CD314 Antibody

Mouse NKG2D/CD314 Antibody

Catalog #: MAB1547
20 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1547-SP
MAB1547-500
MAB1547-100
Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse NKG2D/CD314 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse NKG2D in direct ELISAs and Western blots. Does not cross-react with recombinant human NKG2D.
Source
Monoclonal Rat IgG2B Clone # 191004
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse NKG2D
Phe94-Val232
Accession # O54709
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse NKG2D/CD314 Fc Chimera (Catalog # 139-NK)
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
NK1.1+ mouse splenic NK cells
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.05-0.15 µg/mL of this antibody will block 50% of the binding of 100 ng/mL of biotinylated Recombinant Mouse Rae-1 gamma Fc Chimera to immobilized Recombinant Mouse NKG2D Fc Chimera (Catalog # 139‑NK) coated at 4 µg/mL (100 µL/well). At 2 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2D/CD314

NKG2D, also known as CD314, is a type II transmembrane protein with an extracellular C-type lectin-like domain. It occurs as a disulfide-linked homodimer that associates with the transmembrane DAP10 (DNAX-activator protein 10) adapter protein to deliver an activating signal. This protein shares approximately 25% amino acid sequence identity with a number of other type II lectin-like proteins that are encoded by genes within the natural killer complex on mouse chromosome 6. NKG2D is expressed on NK cells, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion, and on some T cell subsets, where it acts as a co‑stimulatory receptor complementing T cell receptor signaling. Several ligands have now been identified for mouse NKG2D including H60 and Rae 1 alpha, beta, gamma, δ, and epsilon. All of these ligands are cell-surface proteins distantly related to MHC class I. However, they do not bind peptide or associate with beta 2-microglobulin. Ligand expression is up-regulated in many transformed cell lines and also during conditions of stress such as heat shock or viral infection. In vivo, tumor models demonstrate that NKG2D functions in anti-tumor surveillance (1-5).

References
  1. Bauer, S. et al. (1999) Science 285:727.
  2. Wu, J. et al. (1999) Science 285:730.
  3. Diefenbach, A. et al. (2001) Nature 413:165.
  4. Vivier, E. et al. (2002) Curr. Opin. Immunol. 14:306.
  5. NKG2D and its Ligands; www.RnDSystems.com.
Long Name
Natural Killer G2D
Entrez Gene IDs
22914 (Human); 27007 (Mouse); 102120479 (Cynomolgus Monkey)
Alternate Names
CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence

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Citations for Mouse NKG2D/CD314 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. VEGF-A regulated by progesterone governs uterine angiogenesis and vascular remodelling during pregnancy.
    Authors: Kim M, Park HJ, Seol JW et al.
    EMBO Mol Med
  2. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma
    Authors: HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland
    JCI Insight, 2019-07-11;4(13):.
  3. Silencing NKG2D ligand-targeting miRNAs enhances natural killer cell-mediated cytotoxicity in breast cancer
    Authors: Jiaying Shen, Jie Pan, Chengyong Du, Wengong Si, Minya Yao, Liang Xu et al.
    Cell Death & Disease
  4. Natural killer cells act as an extrinsic barrier for in vivo reprogramming
    Authors: Elena Melendez, Dafni Chondronasiou, Lluc Mosteiro, Jaime Martínez de Villarreal, Marcos Fernández-Alfara, Cian J. Lynch et al.
    Development
  5. NKG2D receptor signaling shapes T cell thymic education
    Authors: Perez, C;Plaza-Rojas, L;Boucher, JC;Nagy, MZ;Kostenko, E;Prajapati, K;Burke, B;Reyes, MD;Austin, AL;Zhang, S;Le, PT;Guevara-Patino, JA;
    Journal of leukocyte biology
    Species: Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Combination of anti-CD25 antibody and poly I:C treatment in pregnant NOD mice may be used as "pregnancy-related" type 1 diabetes model
    Authors: A Shimada, K Toda, I Inoue, T Yamada, Y Oikawa
    Journal of diabetes investigation, 2022-05-26;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. NKG2D ligand RAE1? induces generation and enhances the inhibitor function of myeloid-derived suppressor cells in mice
    Authors: L Qian, Y Liu, S Wang, W Gong, X Jia, L Liu, F Ye, J Ding, Y Xu, Y Fu, F Tian
    J. Cell. Mol. Med, 2017-03-09;0(0):.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  8. HkRP3 is a microtubule-binding protein regulating lytic granule clustering and NK cell killing.
    Authors: Ham H, Huynh W, Schoon R, Vale R, Billadeau D
    J Immunol, 2015-03-11;194(8):3984-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
    Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
    J Invest Dermatol, 2015-01-29;135(5):1311-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. NKG2D triggers cytotoxicity in murine epidermal gammadelta T cells via PI3K-dependent, Syk/ZAP70-independent signaling pathway.
    Authors: Ibusuki A, Kawai K, Yoshida S, Uchida Y, Nitahara-Takeuchi A, Kuroki K, Kajikawa M, Ose T, Maenaka K, Kasahara M, Kanekura T
    J Invest Dermatol, 2013-08-20;134(2):396-404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  11. CEACAM1 on activated NK cells inhibits NKG2D-mediated cytolytic function and signaling.
    Authors: Hosomi S, Chen Z, Baker K, Chen L, Huang Y, Olszak T, Zeissig S, Wang J, Mandelboim O, Beauchemin N, Lanier L, Blumberg R
    Eur J Immunol, 2013-06-18;43(9):2473-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation.
    Authors: Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C
    Mucosal Immunol, 2012-06-13;5(0):702.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor gammadelta are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection.
    Authors: Li Z, Zhang C, Zhou Z, Zhang J, Zhang J, Tian Z
    Infect. Immun., 2011-12-05;80(2):565-74.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  14. Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties.
    Authors: Slavuljica I, Busche A, Babic M, Mitrovic M, Gasparovic I, Cekinovic D, Markova Car E, Pernjak Pugel E, Cikovic A, Lisnic VJ, Britt WJ, Koszinowski U, Messerle M, Krmpotic A, Jonjic S
    J. Clin. Invest., 2010-11-22;120(12):4532-45.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
    Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
    Blood, 2009-02-06;113(24):6120-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Two novel NKG2D ligands of the mouse H60 family with differential expression patterns and binding affinities to NKG2D.
    Authors: Takada A, Yoshida S, Kajikawa M, Miyatake Y, Tomaru U, Sakai M, Chiba H, Maenaka K, Kohda D, Fugo K, Kasahara M
    J. Immunol., 2008-02-01;180(3):1678-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  17. Activation of immature hepatic NK cells as immunotherapy for liver metastatic disease.
    Authors: Bahjat KS, Prell RA, Allen HE, Liu W, Lemmens EE, Leong ML, Portnoy DA, Dubensky TW, Brockstedt DG, Giedlin MA
    J. Immunol., 2007-12-01;179(11):7376-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  18. T regulatory cells control numbers of NK cells and CD8alpha+ immature dendritic cells in the lymph node paracortex.
    Authors: Giroux M, Yurchenko E, St-Pierre J, Piccirillo CA, Perreault C
    J. Immunol., 2007-10-01;179(7):4492-502.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. The ectromelia virus virulence factor C15 facilitates early viral spread by inhibiting NK cell contact
    Authors: Elise M. Peauroi, Stephen D. Carro, Luxin Pei, Glennys V. Reynoso, Heather D. Hickman, Laurence C. Eisenlohr
    iScience
  20. Desiccating Stress–Induced Chemokine Expression in the Epithelium Is Dependent on Upregulation of NKG2D/RAE-1 and Release of IFN-gamma in Experimental Dry Eye
    Authors: Terry G. Coursey, Ritu Bohat, Flavia L. Barbosa, Stephen C. Pflugfelder, Cintia S. de Paiva
    The Journal of Immunology

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Mouse NKG2D/CD314 Antibody
By Anonymous on 10/24/2021
Application: WB Sample Tested: Dendritic epidermal T cells Species: Mouse
Western Blot Mouse NKG2D/CD314 Antibody MAB1547