Mouse NKp46/NCR1 Antibody Summary
Glu22-Asn255
Accession # Q8C567
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of NKp46/NCR1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse NKp46/NCR1 Monoclonal Antibody (Catalog # MAB22252) or (B) Rat IgG2AIsotype Control Antibody (Catalog # [[catalogNumber:MAB006)]] followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # [[catalogNumber:F0113]]) and anti-Mouse NK1.1 Alexa Fluor® 488-conjugated Monoclonal Antibody ([[catalogNumber:FAB76141G]]). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKp46/NCR1
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. In human systems, the CD56dimCD16+ subpopulation that makes up the majority of NK cells in the peripheral blood and spleen expresses NKp46 in both resting and activated states (2). The main NK cell population of the lymph node (CD56brightCD16-) expresses low levels of NKp46 in resting cells, but expression is upregulated by IL-2. Mouse NKp46, also known as MAR-1 (3), is a type I transmembrane protein with two extracellular Ig-like domains. It has a positive charge in its transmembrane domain that permits association with the ITAM-bearing signal adapter proteins, CD3 zeta and Fc epsilon RI gamma (4). Studies with neutralizing antibodies indicate that the three NCR are primarily responsible for triggering the NK-mediated lysis of many human tumor cell lines. Blocking any of the NCRs individually resulted in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis was observed if all three receptors were blocked simultaneously (5). NKp46 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (6-8).
- Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
- Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
- Biassoni, R. et al. (1999) Eur. J. Immunol. 29:1014.
- Westgaard, I. et al. (2004) J. Leukoc. Biol. PMID 15356098.
- Pende, D. et al. (1999) J. Exp. Med. 190:1505.
- Arnon, T. et al. (2004) Blood 103:664.
- Arnon, T. et al. (2001) Eur. J. Immunol. 31:2680.
- Mandelboim, O. et al. (2001) Nature 409:1055.
Product Datasheets
Citation for Mouse NKp46/NCR1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Natural Killer-like B Cells Prime Innate Lymphocytes against Microbial Infection.
Authors: Wang S, Xia P, Chen Y, Huang G, Xiong Z, Liu J, Li C, Ye B, Du Y, Fan Z
Immunity, 2016-07-12;45(1):131-44.
Species: Human, Mouse
Sample Types: Whole Tissue
Applications: IHC
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