Mouse OSMR beta PE-conjugated Antibody

Catalog #: FAB662P Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB662P

Detection of OSM R beta in D3 Mouse Cell Line by Flow Cytometry.

1 Image

All OSMR beta Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse OSMR beta PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse OSM R beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CLC, rhCNTF, rhOSM, recombinant mouse (rm) CT-1, rmIL-6, rmIL-11, or rmLIF is observed.

Source

Monoclonal Rat IgG_2A Clone # 118125

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse OSM R beta
Glu24-Leu738
Accession # O70458

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of OSM R beta in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse OSM R beta PE-conjugated Monoclonal Antibody (Catalog # FAB662P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: OSMR beta

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) type I transmembrane protein which contains a 23 aa signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.

References

Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.
Tanaka, M. et al. (1999) Blood 93:804.

Long Name

Oncostatin M Receptor beta

Entrez Gene IDs

9180 (Human); 18414 (Mouse); 310132 (Rat)

Alternate Names

IL-31 receptor subunit beta; IL-31R subunit beta; IL-31RB; IL-31R-beta; Interleukin-31 receptor subunit beta; MGC150627; MGC75127; oncostatin M receptor; oncostatin-M specific receptor beta subunit; oncostatin-M-specific receptor subunit beta; OSM R beta; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1