Mouse PCPE-1 Antibody

Catalog #: MAB2239 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2239
MAB2239-SP

All PCPE-1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PCPE-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PCPE-1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human PCPE-1 is observed. In Western blots, no cross-reactivity with recombinant human PCPE-1 is observed.

Source

Monoclonal Rat IgG₁ Clone # 261845

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PCPE-1
Gln25-Ala468
Accession # Q61398

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse PCPE‑1

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse PCPE‑1, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PCPE-1

Procollagen C‑endopeptidase enhancers, known as PCPEs or PCOLCEs, are secreted extracellular matrix glycoproteins that consist of two CUB domains and one NTR domain. They are known to stimulate enzymatic cleavage of procollagens I‑III by the BMP-1/tolloid family of metalloproteases, also known as procollagen C‑proteinases (1). PCPE‑1 is expressed primarily by interstitial connective tissues such as tendons, calvaria, and skin (2). Although BMP-1/tolloid proteinases are involved in processing of multiple extracellular proteins, the enhancer activity of PCPE-1 is specific to procollagens since it has no effect on BMP‑1/tolloid cleavage of other substrates (3). It is thought that PCPE-1 enhances cleavage of procollagens by binding to the substrate, inducing a conformation change in the substrate (3), although interaction between PCPE-1 and full-length BMP‑1/tolloid proteinases has also been reported (4).

References

Steiglitz, B.M. et al. (2002) J. Biol. Chem. 277:49820.
Kessler, E. et al. (1990) Biochem. Biophys. Res. Commun. 173:81.
Moali, C. et al. (2005) J. Biol. Chem. 280:24188.
Ge, G. et al. (2006) J. Biol. Chem. 281:10786.

Long Name

Procollagen C-Proteinase Enhancer-1

Entrez Gene IDs

5118 (Human); 18542 (Mouse)

Alternate Names

PCOLCE; PCPE; PCPE1; PCPE-1; PCPE1Type I procollagen COOH-terminal proteinase enhancer; procollagen C-endopeptidase enhancer 1; procollagen C-endopeptidase enhancer; Procollagen COOH-terminal proteinase enhancer 1; Procollagen C-proteinase enhancer 1; procollagen, type 1, COOH-terminal proteinase enhancer; Type 1 procollagen C-proteinase enhancer protein