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Mouse PlGF-2 Antibody

Catalog #: MAB465
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB465-SP
MAB465-100
MAB465-500
Product Details
Citations (6)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PlGF-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PlGF-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) PlGF or rhVEGF is observed.
Source
Monoclonal Rat IgG2A Clone # 62526
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2
Ala24-Pro158
Accession # P49764
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse PlGF‑2 (Catalog # 465-PL) under non-reducing conditions only
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.015 - 0.06 μg/mL of this antibody will block 50% of the binding of 4 ng/mL of recombinant mouse PlGF-2 to immobilized recombinant Mouse VEGFR1/Flt-1 Fc Chimera coated at 2 μg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF-2

PlGF is a member of the VEGF family of growth factors, and it binds with high-affinity to VEGF R1. As a result of alternative splicing, three human PlGF mRNAs encode PlGF-1 (PlGF131), PlGF-2 (PlGF152) and PlGF-3 (PlGF203). Normal mouse tissues express only a 158 amino acid precursor that is the equivalent of human PlGF-2.

Long Name
Placenta Growth Factor 2
Entrez Gene IDs
5228 (Human); 18654 (Mouse)
Alternate Names
OORS; PlGF2; PlGF-2

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Citations for Mouse PlGF-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. A Functional Role for VEGFR1 Expressed in Peripheral Sensory Neurons in Cancer Pain
    Authors: Deepitha Selvaraj, Vijayan Gangadharan, Christoph W. Michalski, Martina Kurejova, Sebastian Stösser, Kshitij Srivastava et al.
    Cancer Cell
  2. Blockade of placental growth factor reduces vaso-occlusive complications in murine models of sickle cell disease
    Authors: JM Gu, S Yuan, D Sim, K Abe, P Liu, M Rosenbruch, P Bringmann, K Kauser
    Exp. Hematol., 2018-01-11;0(0):.
    Species: Mouse
    Sample Types: In Vivo, Recombinant Protein
    Applications: Binding Assay, ELISA Development (Detection), Neutralization
  3. Temporal expression of growth factors triggered by epiregulin regulates inflammation development.
    Authors: Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M
    J Immunol, 2015-01-02;194(3):1039-46.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Impaired adipose tissue development in mice with inactivation of placental growth factor function.
    Authors: Lijnen HR, Christiaens V, Scroyen I, Voros G, Tjwa M, Carmeliet P, Collen D
    Diabetes, 2006-10-01;55(10):2698-704.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Synthesis of several chemokines but few cytokines by primed uncommitted precursor CD4 T cells suggests that these cells recruit other immune cells without exerting direct effector functions.
    Authors: Mosmann T
    Eur. J. Immunol., 2004-06-01;34(6):1617-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  6. A critical role of placental growth factor in the induction of inflammation and edema formation.
    Authors: Oura H, Bertoncini J, Velasco P, Brown LF, Carmeliet P, Detmar M
    Blood, 2002-09-05;101(2):560-7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development

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