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Mouse PlGF-2 Antibody

Catalog #: MAB465R Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB465R-100
MAB465R-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PlGF-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PIGF-2 in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG2A Clone # 62526R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PlGF-2
Ala24-Pro158
Accession # P49764
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.015-0.06 μg/mL of this antibody will block 50% of the binding of 4 ng/mL of Recombinant Mouse PlGF-2 to immobilized Recombinant Human Flt-1/Fc Chimera coated at 1 μg/mL (100 µL/well).
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PlGF-2

PlGF is a member of the VEGF family of growth factors, and it binds with high-affinity to VEGF R1. As a result of alternative splicing, three human PlGF mRNAs encode PlGF-1 (PlGF131), PlGF-2 (PlGF152) and PlGF-3 (PlGF203). Normal mouse tissues express only a 158 amino acid precursor that is the equivalent of human PlGF-2.

Long Name
Placenta Growth Factor 2
Entrez Gene IDs
5228 (Human); 18654 (Mouse)
Alternate Names
OORS; PlGF2; PlGF-2

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