Home / Podoplanin / Mouse Podoplanin Antibody

Mouse Podoplanin Antibody

Catalog #: AF3244
44 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3244
AF3244-SP
Detection of Mouse Podoplanin by Western Blot.
2 Images
Product Details
Citations (44)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Podoplanin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Podoplanin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Podoplanin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podoplanin
Gln21-Lys133
Accession # Q546R8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Podoplanin antibody by Western Blot. View Larger

Detection of Mouse Podoplanin by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Podoplanin at approximately 35-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Podoplanin antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Podoplanin in Mouse Kidney. Podoplanin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Podoplanin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3244) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podoplanin

Podoplanin, also known as T1 alpha (T1 alpha ), is a mucin type transmembrane glycoprotein with extensive O-glycosylation. It is specifically expressed by lymphatic endothelial cells but not blood vascular endothelial cells. In addition, non-endothelial cells in numerous normal tissues also express the protein. Within the region used as the immunogen, mouse Podoplanin shares 73.5% and 29% amino acid sequence homology with rat and human Podoplanin, respectively.

Long Name
Lung Type-I Cell Membrane-associated Glycoprotein, Isoform A
Entrez Gene IDs
10630 (Human); 14726 (Mouse); 54320 (Rat)
Alternate Names
36-KD; Aggrus; Gp36; Gp38; GP40; HT1A-1; hT1alpha-1; hT1alpha-2; lung type I cell membrane associated glycoprotein; lung type-I cell membrane-associated glycoprotein (T1A-2); OTS 8; OTS8; PA2.26 antigen; PDPN; Podoplanin; RANDAM-2; T1A; T1A-2; T1-alpha

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse Podoplanin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Hydrogen Sulfide Attenuates Lymphedema Via the Induction of Lymphangiogenesis Through a PI3K/Akt-Dependent Mechanism
    Authors: J Suzuki, Y Shimizu, T Hayashi, Y Che, Z Pu, K Tsuzuki, S Narita, R Shibata, I Ishii, JW Calvert, T Murohara
    Journal of the American Heart Association, 2022-10-26;11(21):e026889.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Sirt3 mediates the benefits of exercise on bone in aged mice
    Authors: Q Li, R Wang, Z Zhang, H Wang, X Lu, J Zhang, AP Kong, XY Tian, HF Chan, AC Chung, JC Cheng, Q Jiang, WY Lee
    Cell Death and Differentiation, 2022-09-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Omega-3 fatty acid epoxides produced by PAF-AH2 in mast cells regulate pulmonary vascular remodeling
    Authors: H Moriyama, J Endo, M Kataoka, Y Shimanaka, N Kono, Y Sugiura, S Goto, H Kitakata, T Hiraide, N Yoshida, S Isobe, T Yamamoto, K Shirakawa, A Anzai, Y Katsumata, M Suematsu, K Kosaki, K Fukuda, H Arai, M Sano
    Nature Communications, 2022-05-31;13(1):3013.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. A High-Fat Diet Activates the BAs-FXR Axis and Triggers Cancer-Associated Fibroblast Properties in the Colon
    Authors: TY Kim, S Kim, Y Kim, YS Lee, S Lee, SH Lee, MN Kweon
    Cellular and Molecular Gastroenterology and Hepatology, 2021-12-29;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Expanded renal lymphatics improve recovery following kidney injury
    Authors: G Baranwal, HA Creed, LM Black, A Auger, AM Quach, R Vegiraju, HE Eckenrode, A Agarwal, JM Rutkowski
    Physiological Reports, 2021-11-01;9(22):e15094.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs
    Authors: L Johnson Ch, H Lahlou, C Steinacher, S Assou, Y Messat, J Dudás, A Edge, B Crespo, M Crosier, C Sergi, A Schrott-Fi, A Zine
    International Journal of Molecular Sciences, 2021-10-07;22(19):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: IF
  7. Mesenchymal stromal cell aging impairs the self-organizing capacity of lung alveolar epithelial stem cells
    Authors: D Chanda, M Rehan, SR Smith, KG Dsouza, Y Wang, K Bernard, D Kurundkar, V Memula, K Kojima, JA Mobley, GA Benavides, V Darley-Usm, YI Kim, JW Zmijewski, JS Deshane, S De Langhe, VJ Thannickal
    Elife, 2021-09-16;10(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Nucleoside-modified VEGFC mRNA induces organ-specific lymphatic growth and reverses experimental lymphedema
    Authors: D Sz?ke, G Kovács, É Kemecsei, L Bálint, K Szoták-Ajt, P Aradi, A Styevkóné, BL Mui, YK Tam, TD Madden, K Karikó, RP Kataru, MJ Hope, D Weissman, BJ Mehrara, N Pardi, Z Jakus
    Nature Communications, 2021-06-08;12(1):3460.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Tertiary lymphoid organs are associated with the progression of kidney damage and regulated by interleukin-17A
    Authors: R Luo, Y Cheng, D Chang, T Liu, L Liu, G Pei, N Zhang, Z Wang, K Guo, W Chen, M Li, L Fan, C Zhang, Y Li, W Dai, M Zuo, Y Xu, Y Yao, S Ge, G Xu
    Theranostics, 2021-01-01;11(1):117-131.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  10. TRPV4 channels are essential for alveolar epithelial barrier function as protection from lung edema
    Authors: J Weber, S Rajan, C Schremmer, YK Chao, G Krasteva-C, M Kannler, AÖ Yildirim, M Brosien, J Schredelse, N Weissmann, C Grimm, T Gudermann, A Dietrich
    JCI Insight, 2020-10-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  11. Lymph node fibroblastic reticular cells deposit fibrosis-associated collagen following organ transplantation
    Authors: X Li, J Zhao, V Kasinath, M Uehara, L Jiang, N Banouni, MM McGrath, T Ichimura, P Fiorina, DR Lemos, SR Shin, CF Ware, JS Bromberg, R Abdi
    J. Clin. Invest., 2020-08-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  12. Alveolar regeneration through a Krt8+ transitional stem cell state that persists in human lung fibrosis
    Authors: M Strunz, LM Simon, M Ansari, JJ Kathiriya, I Angelidis, CH Mayr, G Tsidiridis, M Lange, LF Mattner, M Yee, P Ogar, A Sengupta, I Kukhtevich, R Schneider, Z Zhao, C Voss, T Stoeger, JHL Neumann, A Hilgendorf, J Behr, M O'Reilly, M Lehmann, G Burgstalle, M Königshoff, HA Chapman, FJ Theis, HB Schiller
    Nat Commun, 2020-07-16;11(1):3559.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  13. Keratinocyte-Expressed Podoplanin is Dispensable for Multi-Step Skin Carcinogenesis
    Authors: M Sesarti?, K Ikenberg, SY Yoon, M Detmar
    Cells, 2020-06-24;9(6):.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Characterizing Lymphangiogenesis and Concurrent Inflammation in Adipose Tissue in Response to VEGF-D
    Authors: A Chakrabort, CK Scogin, K Rizwan, TS Morley, JM Rutkowski
    Front Physiol, 2020-04-22;11(0):363.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  15. Overproduction of thrombopoietin by BRAFV600E-mutated mouse hepatocytes and contribution of thrombopoietin to hepatocarcinogenesis
    Authors: H Tanaka, K Horioka, M Yamamoto, M Asari, K Okuda, K Yamazaki, K Shimizu, K Ogawa
    Cancer Sci., 2019-07-30;110(9):2748-2759.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Conditional deletion of E11/podoplanin in bone protects against load-induced osteoarthritis
    Authors: KA Staines, E Ikpegbu, AE Törnqvist, S Dillon, B Javaheri, AK Amin, DN Clements, DJ Buttle, AA Pitsillide, C Farquharso
    BMC Musculoskelet Disord, 2019-07-27;20(1):344.
    Species: Canine, Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. An important role of cutaneous lymphatic vessels in coordinating and promoting anagen hair follicle growth
    Authors: SY Yoon, LC Dieterich, S Karaman, ST Proulx, SB Bachmann, C Sciaroni, M Detmar
    PLoS ONE, 2019-07-25;14(7):e0220341.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. An important role of podoplanin in hair follicle growth
    Authors: SY Yoon, LC Dieterich, C Tacconi, M Sesartic, Y He, L Brunner, O Kwon, M Detmar
    PLoS ONE, 2019-07-23;14(7):e0219938.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. Efficient CD4Cre-Mediated Conditional KRas Expression in Alveolar Macrophages and Alveolar Epithelial Cells Causes Fatal Hyperproliferative Pneumonitis
    Authors: P Chen, S Wang, KS Janardhan, RL Zemans, W Deng, P Karmaus, S Shen, M Sunday, LG Que, MB Fessler, XP Zhong
    J. Immunol., 2019-07-17;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Immune heterogeneity of head and tail pancreatic lymph nodes in non-obese diabetic mice
    Authors: X Li, A Bean, M Uehara, N Banouni, M Ben Nasr, V Kasinath, L Jiang, P Fiorina, R Abdi
    Sci Rep, 2019-07-05;9(1):9778.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival
    Authors: M Uehara, X Li, A Sheikhi, N Zandi, B Walker, B Saleh, N Banouni, L Jiang, F Ordikhani, L Dai, M Yonar, I Vohra, V Kasinath, DP Orgill, A Khademhoss, N Annabi, R Abdi
    Sci Rep, 2019-04-25;9(1):6535.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19
    Authors: L Liu, L Zhao, Y Yang, J Gao, C Hu, B Guo, B Zhu
    Oncol Lett, 2018-08-09;16(4):5013-5019.
  23. Unexpected contribution of lymphatic vessels to promotion of distant metastatic tumor spread
    Authors: Q Ma, LC Dieterich, K Ikenberg, SB Bachmann, J Mangana, ST Proulx, VC Amann, MP Levesque, R Dummer, P Baluk, DM McDonald, M Detmar
    Sci Adv, 2018-08-08;4(8):eaat4758.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing
    Authors: OH Maarouf, M Uehara, V Kasinath, Z Solhjou, N Banouni, B Bahmani, L Jiang, OA Yilmam, I Guleria, SB Lovitch, JL Grogan, P Fiorina, PT Sage, JS Bromberg, MM McGrath, R Abdi
    JCI Insight, 2018-07-12;3(13):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Docetaxel facilitates lymphatic-tumor crosstalk to promote lymphangiogenesis and cancer progression
    Authors: AR Harris, MJ Perez, JM Munson
    BMC Cancer, 2018-07-06;18(1):718.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Salient type 1 interleukin 1 receptor expression in peripheral non-immune cells
    Authors: A Song, L Zhu, G Gorantla, O Berdysz, SA Amici, M Guerau-de-, KM Madalena, JK Lerch, X Liu, N Quan
    Sci Rep, 2018-01-15;8(1):723.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Effects of Orally Ingested Arsenic on Respiratory Epithelial Permeability to Bacteria and Small Molecules in Mice
    Authors: MW Henderson, JH Madenspach, GS Whitehead, SY Thomas, JJ Aloor, KM Gowdy, MB Fessler
    Environ. Health Perspect., 2017-09-28;125(9):097024.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  28. Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response
    Authors: BF Westendorp, NVJA Büller, ON Karpus, WA van Dop, J Koster, R Versteeg, PJ Koelink, CY Snel, S Meisner, JJTH Roelofs, A Uhmann, E Ver Loren, J Heijmans, H Hahn, V Muncan, ME Wildenberg, GR van den Br
    Cell Mol Gastroenterol Hepatol, 2017-09-05;5(1):67-82.e1.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1
    Authors: LA Johnson, S Banerji, W Lawrance, U Gileadi, G Prota, KA Holder, YM Roshorm, T Hanke, V Cerundolo, NW Gale, DG Jackson
    Nat. Immunol., 2017-05-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. CD8+ T cell evasion mandates CD4+ T cell control of chronic gamma-herpesvirus infection
    Authors: CSE Tan, C Lawler, PG Stevenson
    PLoS Pathog., 2017-04-10;13(4):e1006311.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. Differentiation of Club Cells to Alveolar Epithelial Cells In Vitro
    Authors: D Zheng, BS Soh, L Yin, G Hu, Q Chen, H Choi, J Han, VT Chow, J Chen
    Sci Rep, 2017-01-27;7(0):41661.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  32. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
    Authors: Hoeke A Baarsma
    J. Exp. Med, 2016-12-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  33. Postnatal Deletion of Podoplanin in Lymphatic Endothelium Results in Blood Filling of the Lymphatic System and Impairs Dendritic Cell Migration to Lymph Nodes
    Authors: Michael Detmar
    Arterioscler. Thromb. Vasc. Biol., 2016-11-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  34. Type I Interferons Direct Gammaherpesvirus Host Colonization
    PLoS Pathog, 2016-05-25;12(5):e1005654.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Murine junctional adhesion molecules JAM-B and JAM-C mediate endothelial and stellate cell interactions during hepatic fibrosis
    Authors: E Hintermann, M Bayer, J Ehser, M Aurrand-Li, JM Pfeilschif, BA Imhof, U Christen
    Cell Adh Migr, 2016-04-25;10(4):419-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. Aging exacerbates damage and delays repair of alveolar epithelia following influenza viral pneumonia.
    Authors: Yin L, Zheng D, Limmon G, Leung N, Xu S, Rajapakse J, Yu H, Chow V, Chen J
    Respir Res, 2014-09-30;15(0):116.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  37. Effects of adenosine on lymphangiogenesis.
    Authors: Lenoir B, Wagner D, Blacher S, Sala-Newby G, Newby A, Noel A, Devaux Y
    PLoS ONE, 2014-03-20;9(3):e92715.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  38. Key role of microRNA in the regulation of granulocyte macrophage colony-stimulating factor expression in murine alveolar epithelial cells during oxidative stress.
    Authors: Sturrock, Anne, Mir-Kasimov, Mustafa, Baker, Jessica, Rowley, Jesse, Paine, Robert 3
    J Biol Chem, 2013-12-26;289(7):4095-105.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013-12-12;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  40. Regeneration of alveolar type I and II cells from Scgb1a1-expressing cells following severe pulmonary damage induced by bleomycin and influenza.
    Authors: Zheng, Dahai, Limmon, Gino V, Yin, Lu, Leung, Nicola H, Yu, Hanry, Chow, Vincent, Chen, Jianzhu
    PLoS ONE, 2012-10-31;7(10):e48451.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  41. Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage.
    J. Immunol., 2012-07-27;189(5):2181-90.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  42. Th17 cells induce ectopic lymphoid follicles in central nervous system tissue inflammation.
    Authors: Peters A, Pitcher LA, Sullivan JM, Mitsdoerffer M, Acton SE, Franz B, Wucherpfennig K, Turley S, Carroll MC, Sobel RA, Bettelli E, Kuchroo VK
    Immunity, 2011-12-15;35(6):986-96.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  43. The Appearance and Modulation of Osteocyte Marker Expression during Calcification of Vascular Smooth Muscle Cells.
    Authors: Zhu D, Mackenzie NC, Millan JL, Farquharson C, MacRae VE
    PLoS ONE, 2011-05-17;6(5):e19595.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  44. Neuropilin-2 mediates VEGF-C-induced lymphatic sprouting together with VEGFR3.
    Authors: Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivee B, Del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A
    J. Cell Biol., 2010-01-11;188(1):115-30.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse Podoplanin Antibody

There are currently no reviews for this product. Be the first to review Mouse Podoplanin Antibody and earn rewards!

Have you used Mouse Podoplanin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review