Mouse Progranulin/PGRN Antibody

Name: Mouse Progranulin/PGRN Antibody
Brand: R&D Systems
SKU: AF2557
Rating: 5 (6 reviews)

Catalog #: AF2557
35 Citations 6 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2557
AF2557-SP

Detection of Mouse Progranulin/PGRN by Western Blot.

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All Progranulin/PGRN Antibodies

Product Details

Citations (35)

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Supplemental Products

Reviews (6)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Progranulin/PGRN Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Progranulin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Progranulin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Progranulin
Thr18-Leu589
Accession # P28798

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Progranulin/PGRN by Western Blot. Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Progranulin/PGRN in Mouse Splencytes. Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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Progranulin in Mouse Kidney. Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Progranulin/PGRN

Mouse Progranulin, also known as Proepithelin, PC Cell-derived Growth Factor and Acrogranin, is a secreted protein of 572 amino acid residues. It contains seven and one half granulin repeats which are characterized by 6 disulfide bridges. Progranulin can be converted in vivo to granulins by proteolysis. Progranulin is a mitogen that stimulates proliferation and survival of various epithelial, mesenchymal and tumor cells. Several granulins have also been shown to be biologically active and may play a role in inflammation and wound healing. Mouse Progranulin shares 86% and 75% amino acid sequence homology with rat and human Progranulin, respectively.

Entrez Gene IDs

2896 (Human); 14824 (Mouse)

Alternate Names

Acrogranin; CLN11; GEP; GP88; granulin precursor; GRN; PCDGF; PEPI; PGRN; Proepithelin; Progranulin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Progranulin/PGRN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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Hepatocyte-macrophage crosstalk via the PGRN-EGFR axis modulates ADAR1-mediated immunity in the liver
Authors: Gan, WL;Ren, X;Ng, VHE;Ng, L;Song, Y;Tano, V;Han, J;An, O;Xie, J;Ng, BYL;Tay, DJT;Tang, SJ;Shen, H;Khare, S;Chong, KHC;Young, DY;Wu, B;DasGupta, R;Chen, L;
Cell reports
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Investigating the Role and Regulation of GPNMB in Progranulin-deficient Macrophages
Authors: Gillett, DA;Neighbarger, NK;Cole, C;Wallings, RL;Tansey, MG;
bioRxiv : the preprint server for biology
Species: Murine polyomavirus strain A3
Sample Types: Cell Lysates
Applications: Western Blot
Reduced progranulin increases tau and ?-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase
Authors: Takahashi, H;Bhagwagar, S;Nies, SH;Ye, H;Han, X;Chiasseu, MT;Wang, G;Mackenzie, IR;Strittmatter, SM;
Nature communications
Species: Murine polyomavirus strain A3
Sample Types: Whole Tissue
Applications: Immunohistochemistry
Loss of Progranulin Results in Increased Pan-Cathepsin Activity and Reduced LAMP1 Lysosomal Protein
Authors: Anderson, A;Tansey, MG;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Progranulin deficiency results in sex-dependent alterations in microglia in response to demyelination
Authors: Zhang T, Feng T, Wu K et al.
Acta Neuropathologica
AAV-GRN partially corrects motor deficits and ALS/FTLD-related pathology in Tmem106bGrn mice
Authors: Feng T, Minevich G, Liu P et al.
iScience
Decreased Prosaposin and Progranulin in the Cingulate Cortex Are Associated with Schizophrenia Pathophysiology
Authors: Y He, X Zhang, I Flais, P Svenningss
International Journal of Molecular Sciences, 2022-10-10;23(19):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis
Authors: MN Santos, DH Paushter, T Zhang, X Wu, T Feng, J Lou, H Du, SM Becker, R Fragoza, H Yu, F Hu
The Journal of Biological Chemistry, 2022-08-04;0(0):102348.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Progranulin Preserves Autophagy Flux and Mitochondrial Function in Rat Cortical Neurons Under High Glucose Stress
Authors: C Dedert, V Mishra, G Aggarwal, AD Nguyen, F Xu
Frontiers in Cellular Neuroscience, 2022-07-08;16(0):874258.
Species: Rat
Sample Types: Tissue Homogenates, Whole Cells
Applications: ICC, Western Blot
Differential regulation of progranulin derived granulin peptides
Authors: T Zhang, H Du, MN Santos, X Wu, MD Pagan, LJ Trigiani, N Nishimura, T Reinheckel, F Hu
Molecular Neurodegeneration, 2022-02-04;17(1):15.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation
Authors: K Takahashi, S Nakamura, W Otsu, M Shimazawa, H Hara
Journal of Neuroinflammation, 2021-07-25;18(1):164.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot

Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
Authors: J Frew, HB Nygaard
Acta neuropathologica communications, 2021-04-01;9(1):57.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot

Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice
Authors: M Murakoshi, T Gohda, E Adachi, S Ichikawa, S Hagiwara, Y Suzuki
Scientific Reports, 2021-01-13;11(1):1194.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Effects of progranulin on the pathological conditions in experimental myocardial infarction model
Authors: T Sasaki, M Shimazawa, H Kanamori, Y Yamada, A Nishinaka, Y Kuse, G Suzuki, T Masuda, S Nakamura, M Hosokawa, S Minatoguch, H Hara
Sci Rep, 2020-07-16;10(1):11842.
Species: Mouse
Sample Types: Tissue Lysate, Whole Tissue
Applications: IF, Western Blot

Coupling of terminal differentiation deficit with neurodegenerative pathology in Vps35-deficient pyramidal neurons
Authors: FL Tang, L Zhao, Y Zhao, D Sun, XJ Zhu, L Mei, WC Xiong
Cell Death Differ., 2020-01-06;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Progranulin deficiency exacerbates spinal cord injury by promoting neuroinflammation and cell apoptosis in mice
Authors: C Wang, L Zhang, JC Ndong, A Hettinghou, G Sun, C Chen, C Zhang, R Liu, CJ Liu
J Neuroinflammation, 2019-11-27;16(1):238.
Species: Mouse
Sample Types: Tissue homogena
Applications: Western Blot

Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling
Authors: F Wei, Z Jiang, H Sun, J Pu, Y Sun, M Wang, Q Tong, Y Bi, X Ma, GF Gao, J Liu
PLoS Pathog., 2019-10-04;15(10):e1008062.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Progranulin deficiency leads to reduced glucocerebrosidase activity
Authors: X Zhou, DH Paushter, MD Pagan, D Kim, M Nunez Sant, RL Lieberman, HS Overkleeft, Y Sun, MB Smolka, F Hu
PLoS ONE, 2019-07-10;14(7):e0212382.
Species: Mouse
Sample Types: Whole Cells
Applications: Western Blot

Genetic Regulation of Neuronal Progranulin Reveals a Critical Role for the Autophagy-Lysosome Pathway
Authors: LP Elia, AR Mason, A Alijagic, S Finkbeiner
J. Neurosci., 2019-01-29;0(0):.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Detection)

Microglia increases the proliferation of retinal precursor cells during postnatal development
Authors: Y Kuse, K Ohuchi, S Nakamura, H Hara, M Shimazawa
Mol. Vis., 2018-07-30;24(0):536-545.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot

Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity
Authors: AM Nicholson, X Zhou, RB Perkerson, TM Parsons, J Chew, M Brooks, M DeJesus-He, NA Finch, BJ Matchett, A Kurti, KR Jansen-Wes, E Perkerson, L Daughrity, M Castanedes, L Rousseau, V Phillips, F Hu, TF Gendron, ME Murray, DW Dickson, JD Fryer, L Petrucelli, R Rademakers
Acta Neuropathol Commun, 2018-05-31;6(1):42.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum
Authors: N Uesaka, M Abe, K Konno, M Yamazaki, K Sakoori, T Watanabe, TH Kao, T Mikuni, M Watanabe, K Sakimura, M Kano
Neuron, 2018-02-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology
Authors: K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa
Nat Commun, 2018-01-30;9(1):433.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis
Authors: AE Arrant, VC Onyilo, DE Unger, ED Roberson
J. Neurosci., 2018-01-29;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot

Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle
Authors: H Sugihara, K Miyaji, K Yamanouchi, T Matsuwaki, M Nishihara
J. Vet. Med. Sci., 2017-12-18;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease
Authors: JM Van Kampen, DG Kay
PLoS ONE, 2017-08-24;12(8):e0182896.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot

Lysosomal processing of progranulin
Authors: X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu
Mol Neurodegener, 2017-08-23;12(1):62.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Progranulin functions as a cathepsin D chaperone to stimulate axonal outgrowth in vivo
Authors: S Beel, M Moisse, M Damme, L De Muynck, W Robberecht, L Van Den Bo, P Saftig, P Van Damme
Hum. Mol. Genet., 2017-08-01;26(15):2850-2863.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice
Authors: TL Petkau, J Blanco, BR Leavitt
Neurobiol. Dis., 2017-06-21;106(0):14-22.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina
Authors: Y Kuse, K Tsuruma, S Sugitani, H Izawa, Y Ohno, M Shimazawa, H Hara
Sci Rep, 2016-03-31;6(0):23811.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IHC-Fr, Western Blot

Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells.
Authors: Luo L, Lu L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J
Mol Med Rep, 2014-03-06;9(5):1675-80.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot

p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
Biochem Biophys Res Commun, 2012-12-01;430(1):419-24.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Progranulin regulates neuronal outgrowth independent of sortilin.
Authors: Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L
Mol Neurodegener, 2012-07-10;7(0):33.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins.
Authors: Almeida S, Zhou L, Gao FB
PLoS ONE, 2011-10-18;6(10):e26454.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
Authors: Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S
J. Pathol., 2009-10-01;219(2):173-81.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot