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Mouse RANK/TNFRSF11A Antibody

Catalog #: AF692
10 Citations Datasheet / COA / SDS
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AF692
AF692-SP
Mouse RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation in Mouse Splenocytes.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse RANK/TNFRSF11A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse RANK/TNFRSF11A in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human RANK is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse RANK/TNFRSF11A
Gln30-Pro213
Accession # O35305
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse RANK/TNFRSF11A Fc Chimera (Catalog # 692-RK)
Immunohistochemistry
5-15 µg/mL
See below
Agonist Activity
0.1-0.4 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Agonist Activity Mouse RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation antibody in Mouse Splenocytes. View Larger

Mouse RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation in Mouse Splenocytes. Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody induces osteoclast differentiationon mouse splenocytes in the presence of 20 ng/mL recombinant mouse M-CSF (Catalog # 416-ML), in a dose-dependent manner, as measured by the tartrate-resistant acid phosphatase (TRAP) solution assay. The ED50 for this effect is typically 0.1-0.4 µg/mL.

Immunohistochemistry RANK/TNFRSF11A antibody in Rat Embryo by Immunohistochemistry (IHC-Fr). View Larger

RANK/TNFRSF11A in Rat Embryo. RANK/TNFRSF11A was detected in immersion fixed frozen sections of rat embryo (cartillage primordium, 7 days p.c.) using 10 µg/mL Goat Anti-Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF692) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RANK/TNFRSF11A

RANK (receptor activator of NF-kappa B, also known as TRANCE receptor, osteoclast differentiation factor receptor [ODFR] and TNFRSF11A) is a member of the tumor necrosis factor receptor family. The full length mouse RANK cDNA encodes a type I transmembrane protein of 625 amino acids (aa) with a predicted 184 aa extracellular domain and a 391 aa cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% aa identity in their extracellular domains. RANK is widely expressed with the highest levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. RANK is expressed in dendritic cells. In activated human peripheral blood T lymphocytes, RANK expression is induced by IL-4 and TGF-beta. Multiple tumor necrosis factor receptor-associated factors (TRAFs) are involved in the signaling of RANK. TRANCE (TNF-related activation-induced cytokines, also known as RANK ligand [RANKL], osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]) is the ligand for RANK. The biological functions mediated through RANK include activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Soluble RANK is able to block TRANCE induced biological activity.

References
  1. Anderson, D.M. et al. (1997) Nature 390:175.
  2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
Long Name
Receptor Activator of NF-kappa-B
Entrez Gene IDs
8792 (Human); 21934 (Mouse)
Alternate Names
CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator

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Citations for Mouse RANK/TNFRSF11A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Mechanosensitive hormone signaling promotes mammary progenitor expansion and breast cancer risk
    Authors: Northey, JJ;Hayward, MK;Yui, Y;Stashko, C;Kai, F;Mouw, JK;Thakar, D;Lakins, JN;Ironside, AJ;Samson, S;Mukhtar, RA;Hwang, ES;Weaver, VM;
    Cell stem cell
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation
    Authors: SG Romeo, KM Alawi, J Rodrigues, A Singh, AP Kusumbe, SK Ramasamy
    Nat. Cell Biol., 2019-04-01;21(4):430-441.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis
    Authors: J Jhun, SH Lee, SY Kim, J Ryu, JY Kwon, HS Na, K Jung, SJ Moon, ML Cho, JK Min
    J Transl Med, 2019-03-15;17(1):84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity.
    Authors: Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V
    Autophagy, 2014-10-30;10(11):2036-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice.
    Authors: Shimamura M, Nakagami H, Osako M, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R
    Proc Natl Acad Sci U S A, 2014-05-20;111(22):8191-6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. MHC class II transactivator is an in vivo regulator of osteoclast differentiation and bone homeostasis co-opted from adaptive immunity.
    Authors: Benasciutti E, Mariani E, Oliva L, Scolari M, Perilli E, Barras E, Milan E, Orfanelli U, Fazzalari N, Campana L, Capobianco A, Otten L, Particelli F, Acha-Orbea H, Baruffaldi F, Faccio R, Sitia R, Reith W, Cenci S
    J Bone Miner Res, 2014-02-01;29(2):290-303.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.
    Authors: Danzl N, Jeong S, Choi Y, Alexandropoulos K
    PLoS ONE, 2014-01-21;9(1):e86129.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  8. Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors.
    Authors: Baik S, Jenkinson E, Lane P, Anderson G, Jenkinson W
    Eur J Immunol, 2013-02-11;43(3):589-94.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Neutralization
  9. The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse.
    Authors: Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP
    Dev. Biol., 2009-01-23;328(1):127-39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
    Authors: Kovacic N, Lukic IK, Grcevic D, Katavic V, Croucher P, Marusic A
    J. Immunol., 2007-03-15;178(6):3379-89.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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