Mouse/Rat Apolipoprotein H/ApoH Antibody

Catalog #: AF6575
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6575
AF6575-SP

Detection of Rat and Mouse Apolipoprotein H/ApoH by Western Blot.

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Mouse/Rat Apolipoprotein H/ApoH Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat Apolipoprotein H/ApoH in Western blots and detects recombinant mouse Apolipoprotein H/ApoH in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Apolipoprotein H/ApoH is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Apolipoprotein H/ApoH
Gly20-Cys345
Accession # Q01339

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

0.25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat and Mouse Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of rat liver tissue and mouse serum. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse/Rat Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein H/ApoH at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein H/ApoH

Apolipoprotein H (ApoH), also known as beta 2‑Glycoprotein I/ beta 2-GPI, is a variably glycosylated member of the complement control superfamily of proteins with a molecular weight of aproximately 50 kDa (1, 2). Mature mouse ApoH consists of four tandem Sushi/SCR repeats followed by one Sushi-like repeat (3, 4). Mature mouse ApoH shares 76% and 42% aa sequence identity with human and rat ApoH, respectively. Hepatocyte-derived ApoH binds directly to negatively charged phospholipids (5). It circulates as a component of lipoprotein particles and as a lipid-free serum protein (6). ApoH also associates with liposomes and apoptotic cell debris, thereby enabling their renal clearance via Megalin uptake (7, 8). Circulating levels of ApoH are postively correlated with triglyceride-rich lipoprotein (VLDL) components in type II diabetes (9). ApoH inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows procoagulant activity by inhibiting the activation of Protein C (10, 11). ApoH can be cleaved by Plasmin at Lys317‑Thr318, an action that is enhanced by heparin (12, 13). ApoH cleavage reduces its ability to bind phospholipids and inhibit Factor XI activation but confers the ability to bind Plasminogen (10, 12, 14). Cleaved ApoH also demonstrates antiangiogenic activity, whereas intact ApoH does not (14). The production of antibodies against ApoH is a hallmark of Antiphospholipid Syndrome (APS), an autoimmune disorder that leads to hypercoagulability and recurrent miscarriages (15). ApoH binds to the surface antigen of Hepatitis B Virus and is associated with the development of HBV-induced hepatocellular carcinoma (6, 16).

References

Crook, M.A. et al. (2010) Atherosclerosis 209:32.
Miyakis, S. et al. (2004) Thromb. Res. 114:335.
Nonaka, M. et al. (1992) Genomics 13:1082.
Lozier, J. et al. (1984) Proc. Natl. Acad. Sci. 81:3640.
Wurm, H. (1984) Int. J. Biochem. 16:511.
Mehdi, H. et al. (1994) J. Virol. 68:2415.
Chonn, A. et al. (1995) J. Biol. Chem. 270:25845.
Moestrup, S.K. et al. (1998) J. Clin. Invest. 102:902.
Castro, A. et al. (2010) Atherosclerosis 209:201.
Shi, T. et al. (2004) Proc. Natl. Acad. Sci. 101:3939.
Mori, T. et al. (1996) Thromb. Haemost. 75:49.
Hunt, J. et al. (1993) Proc. Natl. Acad. Sci. 90:2141.
Guerin, J. et al. (2002) J. Biol. Chem. 277:2644.
Sakai, T. et al. (2007) Am. J. Pathol. 171:1659.
Adams, M. (2008) Semin. Thromb. Haemost. 34:251.
Jing, X. et al. (2010) J. Cancer Res. Clin. Oncol. 16:1671.

Entrez Gene IDs

350 (Human); 11818 (Mouse); 287774 (Rat)

Alternate Names

Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; APOH; apo-H; apolipoprotein H (beta-2-glycoprotein I); Apolipoprotein H; B2G1; B2GP1; B2GPI; Beta; beta(2)GPI; beta-2-glycoprotein 1; Beta-2-glycoprotein I; BG

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Citation for Mouse/Rat Apolipoprotein H/ApoH Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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