Mouse/Rat CD34 Antibody Summary
Thr35-Thr287
Accession # Q64314
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse and Rat CD34 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue and rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6518) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD34 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Detection of CD34 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6518, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD34
Mouse and rat CD34 is a 80 -100 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, CD34+ stem cells and renal mesangial cells. Mature mouse CD34 is a 348 amino acid (aa) type I transmembrane protein (aa 35-382). It contains a 253 aa extracellular region (aa 35-287), and a 74 aa cytoplasmic domain that undergoes Tyr phosphorylation. Mouse and rat CD34 also is likely to undergo membrane cleavage that will generate a 54 kDa soluble form. Over aa 35-287, mouse CD34 shares 57% and 82% aa identity with human and rat Cd34, respectively.
Product Datasheets
Citations for Mouse/Rat CD34 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Combination human umbilical cord perivascular and endothelial colony forming cell therapy for ischemic cardiac injury
Authors: Iqbal F, Johnston A, Wyse B et al.
npj Regenerative Medicine
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The Effect of Electronic-Cigarette Vaping on Cardiac Function and Angiogenesis in Mice
Authors: H Shi, X Fan, A Horton, ST Haller, DJ Kennedy, IT Schiefer, L Dworkin, CJ Cooper, J Tian
Sci Rep, 2019-03-11;9(1):4085.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
Authors: R El-Habta, M Sloniecka, PJ Kingham, LJ Backman
Stem Cell Res Ther, 2018-12-20;9(1):352.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry -
Fibrocyte migration, differentiation and apoptosis during the corneal wound healing response to injury
Authors: L Lassance, GK Marino, CS Medeiros, S Thangavadi, SE Wilson
Exp. Eye Res., 2018-02-24;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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