Mouse/Rat CD47 N-terminal IgV-like Extracellular Domain Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse CD47 by Western Blot. Western blot shows lysates of mouse ovary tissue and mouse thymus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat CD47 N-terminal IgV-like Extracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1866) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). Specific bands were detected for CD47 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Rat CD47 by Western Blot. Western blot shows lysates of rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat CD47 N-terminal IgV-like Extracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1866) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for CD47 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of CD47 in Mouse Spleen. CD47 was detected in immersion fixed paraffin-embedded sections of Mouse Spleen using Goat Anti-Mouse/Rat CD47 N-terminal IgV-like Extracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1866) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Cell surface and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: CD47
CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a 40-60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Mouse CD47 is an integral membrane protein that consists of a 122 amino acid (aa) extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and a 16 aa C-terminal cytoplasmic tail (3). Alternate splicing of mouse CD47 generates an additional isoform with an insertion of 21 aa following the Ig-like domain (3). Within the N-terminal ECD, mouse CD47 shares 63% and 84% aa sequence identity with human and rat CD47, respectively. A portion of the N-terminal ECD can by shed from smooth muscle cells by MMP-2-mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5-8). CD47 associates in cis with Fas on T cells and enhances Fas-mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9-11). CD47 also associates in cis with Integrins alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and alpha 2 beta 1 which can positively or negatively modulate Integrin-mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin-1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP-1 induces giant cell formation and osteoclast differentiation (15).
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- Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
- Lindberg, F.P. et al. (1993) J. Cell Biol. 123:485.
- Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.
- Oldenborg, P.-A. et al. (2000) Science 288:2051.
- Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.
- Stefanidakis, M. et al. (2008) Blood 112:1280.
- de Vries, H.E. et al. (2002) J. Immunol. 168:5832.
- Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.
- Avice, M.-N. et al. (2001) J. Immunol. 167:2459.
- Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.
- Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.
- Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.
- Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
- Kukreja, A. et al. (2009) Blood 114:3413.
Product Datasheets
Citations for Mouse/Rat CD47 N-terminal IgV-like Extracellular Domain Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection
Authors: Moon, S;Han, S;Jang, IH;Ryu, J;Rha, MS;Cho, HJ;Yoon, SS;Nam, KT;Kim, CH;Park, MS;Seong, JK;Lee, WJ;Yoon, JH;Chung, YW;Ryu, JH;
Nature communications
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: Immunohistochemistry, Western Blot -
CD47 masks pro-phagocytic ligands in cis on tumor cells to suppress antitumor immunity
Authors: Tang, Z;Zhong, MC;Qian, J;Galindo, CC;Davidson, D;Li, J;Zhao, Y;Hui, E;Veillette, A;
Nature immunology
Species: Mouse
Sample Types: Protein
Applications: Immunoprecipitation, Western Blot -
Loss of microglial SIRP&alpha promotes synaptic pruning in preclinical models of neurodegeneration
Authors: X Ding, J Wang, M Huang, Z Chen, J Liu, Q Zhang, C Zhang, Y Xiang, K Zen, L Li
Nature Communications, 2021-04-01;12(1):2030.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
CD47 prevents the elimination of diseased fibroblasts in scleroderma
Authors: T Lerbs, L Cui, ME King, T Chai, C Muscat, L Chung, R Brown, K Rieger, T Shibata, G Wernig
JCI Insight, 2020-08-20;5(16):.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo
Authors: V Azcutia, M Kelm, AC Luissint, K Boerner, S Flemming, M Quiros, G Newton, A Nusrat, FW Luscinskas, CA Parkos
Mucosal Immunol, 2020-06-19;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Epithelial CD47 is critical for mucosal repair in the murine intestine in vivo
Authors: M Reed, AC Luissint, V Azcutia, S Fan, MN O'Leary, M Quiros, J Brazil, A Nusrat, CA Parkos
Nat Commun, 2019-11-01;10(1):5004.
Species: Mouse
Sample Types: Cells
Applications: IF, Western Blot -
Radiation-induced loss of cell surface CD47 enhances immune-mediated clearance of human papillomavirus-positive cancer.
Authors: Vermeer D, Spanos W, Vermeer P, Bruns A, Lee K, Lee J
Int J Cancer, 2013-02-12;133(1):120-9.
Species: Human, Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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CD47 expression is highly variable in PBMC of mice exposed to different inflammatory and anti-inflammatory stimuli
