Home / Complement Component C3d / Mouse/Rat Complement Component C3d Antibody

Mouse/Rat Complement Component C3d Antibody

Catalog #: AF2655
27 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2655
AF2655-SP
Complement Component C3d in Mouse Kidney.
2 Images
Product Details
Citations (27)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Product Datasheets Related Research Areas

Mouse/Rat Complement Component C3d Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse Complement Component C3d in direct ELISAs and Western blots. Detects mouse and rat Complement Component C3d in immunohistochemistry.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Complement Component C3d
His1002-Arg1303 (Cys1010Ser)
Accession # P01027
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Complement Component C3d
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Complement Component C3d antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Complement Component C3d in Mouse Kidney. Complement Component C3d was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Complement Component C3d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2655) overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to basement membrane. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Complement Component C3d antibody in Rat Kidney by Immunohistochemistry (IHC-P). View Larger

Complement Component C3d in Rat Kidney. Complement Component C3d was detected in perfusion fixed paraffin-embedded sections of rat kidney using Goat Anti-Mouse Complement Component C3d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2655) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Complement Component C3d

Entrez Gene IDs
718 (Human)
Alternate Names
AHUS5;ARMD9;ASP;C3a;C3b;Complement C3;CPAMD1;HEL-S-62p; Complement Component C3d

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Citations for Mouse/Rat Complement Component C3d Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Gliosis-dependent expression of complement factor H truncated variants attenuates retinal neurodegeneration following ischemic injury
    Authors: Biber, J;Jabri, Y;Glänzer, S;Dort, A;Hoffelner, P;Schmidt, CQ;Bludau, O;Pauly, D;Grosche, A;
    Journal of neuroinflammation
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis
    Authors: X Sun, Y Ni, Q Lu, Y Liang, M Gu, X Xue, C Dai
    Cell Death & Disease, 2022-12-09;13(12):1031.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Microglia Depletion from Primary Glial Cultures Enables to Accurately Address the Immune Response of Astrocytes
    Authors: M Van Zeller, AM Sebastião, CA Valente
    Biomolecules, 2022-05-04;12(5):.
    Species: Mouse
    Sample Types: Cell Lyates
    Applications: Western Blot
  4. Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice
    Authors: T Wan, W Zhu, Y Zhao, X Zhang, R Ye, M Zuo, P Xu, Z Huang, C Zhang, Y Xie, X Liu
    Nature Communications, 2022-03-03;13(1):1134.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. C4d Deposition after Allogeneic Renal Transplantation in Rats Is Involved in Initial Apoptotic Cell Clearance
    Authors: S Reuter, D Kentrup, A Grabner, G Köhler, K Buscher, B Edemir
    Cells, 2021-12-10;10(12):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemia-reperfusion injury
    Authors: AK Bongoni, IB Vikstrom, JL McRae, EJ Salvaris, N Fisicaro, MJ Pearse, S Wymann, T Rowe, AB Morelli, MP Hardy, PJ Cowan
    Scientific Reports, 2021-11-08;11(1):21873.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Role of uromodulin and complement activation in the progression of kidney disease
    Authors: F Shen, M Liu, F Pei, L Yu, X Yang
    Oncology Letters, 2021-10-13;22(6):829.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IF
  8. Complement factor B in high glucose-induced podocyte injury and diabetic kidney disease
    Authors: Q Lu, Q Hou, K Cao, X Sun, Y Liang, M Gu, X Xue, AZ Zhao, C Dai
    JCI Insight, 2021-10-08;6(19):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. C-reactive protein protects against acetaminophen-induced liver injury by preventing complement overactivation
    Authors: HY Li, ZM Tang, Z Wang, JM Lv, XL Liu, YL Liang, B Cheng, N Gao, SR Ji, Y Wu
    Cellular and Molecular Gastroenterology and Hepatology, 2021-09-16;0(0):.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture
  10. Inducing regulated necrosis and shifting macrophage polarization with anti-EMMPRIN antibody (161-pAb) and complement factors
    Authors: N Hijaze, M Ledersnaid, E Simanovich, S Kassem, MA Rahat
    J Leukoc Biol, 2020-11-17;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Cell-Type-Specific Complement Profiling in the ABCA4-/- Mouse Model of Stargardt Disease
    Authors: Y Jabri, J Biber, N Diaz-Lezam, A Grosche, D Pauly
    Int J Mol Sci, 2020-11-11;21(22):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  12. Neuron-Derived Estrogen is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain
    Authors: Y Lu, GR Sareddy, J Wang, Q Zhang, FL Tang, UP Pratap, RR Tekmal, RK Vadlamudi, DW Brann
    J. Neurosci., 2020-08-19;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Protein, Whole Tissue
    Applications: IHC, Western Blot
  13. Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype
    Authors: B Hou, Y Zhang, P Liang, Y He, B Peng, W Liu, S Han, J Yin, X He
    Cell Death Dis, 2020-05-15;11(5):377.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  14. Glomerular Injury Is Exacerbated in Lupus-Prone MRL/lpr Mice Treated with a Protease-Activated Receptor 2 Antagonist
    Authors: R Itto, Y Oe, K Imaruoka, E Sato, A Sekimoto, S Yamakage, S Kumakura, H Sato, S Ito, N Takahashi
    Tohoku J. Exp. Med., 2019-10-01;249(2):127-133.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Complement Inhibitor CRIg/FH Ameliorates Renal Ischemia Reperfusion Injury via Activation of PI3K/AKT Signaling
    Authors: C Hu, L Li, P Ding, L Li, X Ge, L Zheng, X Wang, J Wang, W Zhang, N Wang, H Gu, F Zhong, M Xu, R Rong, T Zhu, W Hu
    J. Immunol., 2018-11-14;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection
    Authors: A Alawieh, M Andersen, DL Adkins, S Tomlinson
    J. Neurosci., 2018-06-19;38(29):6527-6545.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Identifying the role of complement in triggering neuroinflammation after traumatic brain injury
    Authors: A Alawieh, EF Langley, S Weber, D Adkins, S Tomlinson
    J. Neurosci., 2018-02-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. C3d regulates immune checkpoint blockade and enhances antitumor immunity
    Authors: JL Platt, I Silva, SJ Balin, AR Lefferts, E Farkash, TM Ross, MC Carroll, M Cascalho
    JCI Insight, 2017-05-04;2(9):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  19. Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-beta Signaling in the Retinal Pigment Epithelium.
    Authors: Li Y, Song D, Song Y, Zhao L, Wolkow N, Tobias J, Song W, Dunaief J
    J Biol Chem, 2015-03-23;290(19):11918-34.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. The epidermal growth factor receptor is a regulator of epidermal complement component expression and complement activation.
    Authors: Abu-Humaidan A, Ananthoju N, Mohanty T, Sonesson A, Alberius P, Schmidtchen A, Garred P, Sorensen O
    J Immunol, 2014-03-03;192(7):3355-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. Soluble CR1 therapy improves complement regulation in C3 glomerulopathy.
    Authors: Zhang Y, Nester C, Holanda D, Marsh H, Hammond R, Thomas L, Meyer N, Hunsicker L, Sethi S, Smith R
    J Am Soc Nephrol, 2013-08-01;24(11):1820-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Targeting complement component 5a promotes vascular integrity and limits airway remodeling.
    Authors: Khan M, Maasch C, Vater A, Klussmann S, Morser J, Leung L, Atkinson C, Tomlinson S, Heeger P, Nicolls M
    Proc Natl Acad Sci U S A, 2013-03-25;110(15):6061-6.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Donor brain death exacerbates complement-dependent ischemia/reperfusion injury in transplanted hearts.
    Authors: Atkinson, Carl, Floerchinger, Bernhard, Qiao, Fei, Casey, Sarah, Williamson, Tucker, Moseley, Ellen, Stoica, Serban, Goddard, Martin, Ge, Xupeng, Tullius, Stefan G, Tomlinson, Stephen
    Circulation, 2013-02-26;127(12):1290-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. The alternative complement pathway propagates inflammation and injury in murine ischemic stroke.
    Authors: Elvington, Andrew, Atkinson, Carl, Zhu, Hong, Yu, Jin, Takahashi, Kazue, Stahl, Gregory, Kindy, Mark S, Tomlinson, Stephen
    J Immunol, 2012-10-01;189(9):4640-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. Pathogenic natural antibodies propagate cerebral injury following ischemic stroke in mice.
    Authors: Elvington A, Atkinson C, Kulik L
    J. Immunol., 2011-12-23;188(3):1460-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  26. Age-related retinopathy in NRF2-deficient mice.
    Authors: Zhao Z, Chen Y, Wang J
    PLoS ONE, 2011-04-29;6(4):e19456.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice.
    Authors: Leung VW, Yun S, Botto M, Mason JC, Malik TH, Song W, Paixao-Cavalcante D, Pickering MC, Boyle JJ, Haskard DO
    Am. J. Pathol., 2009-09-03;175(4):1757-67.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

FAQs

  1. Does Mouse/Rat Complement Component C3d Antibody, Catalog # AF2655, detect/cross-react with C3b or intact C3?

    • Since complement component C3d is derived from C3b, we expect AF2655 to recognize C3b and C3d in western blot. We have not tested AF2655 against intact C3, but there is published literature that shows that intact C3 is not recogniozed by AF2655 in IHC.

  2. For Mouse/Rat Complement Component C3d Antibody, Catalog # AF2655, what is the expected molecular weight in Western Blot of the recombinant mouse and natural Complement Component C3d?

    • The mouse Complement Component C3d is expected to have a moleculat weight of  ~34 kDa.

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Mouse Complement Component C3d Antibody
By Anonymous on 12/19/2016
Application: ICC/IF Sample Tested: A549 human lung carcinoma cell line Species: Human