Mouse/Rat Growth Hormone Biotinylated Antibody

Catalog #: BAF1566 Datasheet / COA / SDS

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BAF1566

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Mouse/Rat Growth Hormone Biotinylated Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects rat Growth Hormone in Western blots. In this format, approximately 10% cross-reactivity with recombinant human Growth Hormone is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat Growth Hormone
Leu27-Phe216
Accession # P01244

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat Growth Hormone

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Growth Hormone in Rat Pituitary. Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

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Growth Hormone in Mouse Embryo. Growth Hormone was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Rat Growth Hormone Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone

Growth Hormone, also named somatotropin, belongs to the somatotropin/prolactin superfamily. It is synthesized in the anterior pituitary gland. Growth Hormone regulates body growth by stimulating the secretion of IGF-I by liver and other tissues.

Entrez Gene IDs

2688 (Human)

Alternate Names

GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin