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Mouse/Rat HO-1/HMOX1/HSP32 Antibody

Catalog #: AF3169
3 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3169
AF3169-SP
Detection of Mouse/Rat HO‑1/HMOX1/HSP32 by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat HO-1/HMOX1/HSP32 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat HO-1/HMOX1/HSP32 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat HO-1/HMOX1
Met1-Met289
Accession # P06762
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat HO‑1/HMOX1/HSP32 antibody by Western Blot. View Larger

Detection of Mouse/Rat HO‑1/HMOX1/HSP32 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3169) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HO-1/HMOX1/HSP32

Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Rat HO-1 shares 93% amino acid sequence identity with mouse HO-1.

Long Name
Heme Oxygenase 1
Entrez Gene IDs
3162 (Human); 24451 (Mouse); 15368 (Rat)
Alternate Names
bK286B10; EC 1.14.99.3; heat shock protein, 32-kD; heme oxygenase (decycling) 1; HMOX1; HO; HO1; HO-1; HO-1heme oxygenase 1; HSP32

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Citations for Mouse/Rat HO-1/HMOX1/HSP32 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms
    Authors: MS Abdelfatta, MIY Elmallah, HY Ebrahim, RS Almeer, RMA Eltanany, AE Abdel Mone
    PLoS ONE, 2019-06-13;14(6):e0216737.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Royal jelly attenuates cadmium-induced nephrotoxicity in male mice
    Authors: RS Almeer, GI AlBasher, S Alarifi, S Alkahtani, D Ali, AE Abdel Mone
    Sci Rep, 2019-04-09;9(1):5825.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke
    Evid Based Complement Alternat Med, 2016-11-28;2016(0):1698379.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Mouse/Rat HO-1/HMOX1/HSP32 Antibody

Average Rating: 4 (Based on 1 Review)

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Mouse/Rat HO-1/HMOX1/HSP32 Antibody
By Anonymous on 06/01/2016
Application: WB Sample Tested: Colon tissue,Kidney tissue,Liver tissue,mouse embryonic fibroblasts,Small intestine tissue Species: Human, Mouse, Rat

Primary ab dilution 1:2000 (better with 1:1000)
40 ug protein per lane

Western Blot Mouse/Rat HO-1/HMOX1/HSP32 Antibody AF3169