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Mouse, Rat, Porcine, & Canine IGF-II/IGF2 ELISA Kit - Quantikine

Catalog #: MG200
8 Citations Datasheet / COA / SDS
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MG200
Mouse/Rat/Porcine/Canine IGF-II ELISA Standard Curve
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sensitivity
1.87 pg/mL
Assay Range
12.5 - 800 pg/mL (Serum-free Cell Culture Supern, Mouse Serum, Rat Serum, Canine Serum, Porcine Serum)
Specificity
Natural and recombinant IGF-II.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine® Mouse/Rat/Porcine/Canine IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure IGF-II in serum-free cell culture supernates and serum. It contains E. coli-expressed recombinant IGF-II and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural IGF-II.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Serum-free Cell Culture Supernates, Serum, Mouse Serum, Rat Serum, Canine Serum, Porcine Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 46.1 81.7 285 43.5 80.7 275
Standard Deviation 2.14 3.41 10.8 2.58 4.97 16.8
CV% 4.6 4.2 3.8 5.9 6.2 6.1

Recovery

The recovery of IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Serum (n=4) 105 86-124
Rat Serum (n=4) 84 78-89
Serum-free Cell Culture Media (n=4) 92 84-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat/Porcine/Canine IGF-II Linearity

Scientific Data

N/A Mouse/Rat/Porcine/Canine IGF-II ELISA Standard Curve View Larger

Mouse/Rat/Porcine/Canine IGF-II ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 degreesC. Do not use past expiration date.

Background: IGF-II/IGF2

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity. The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

Long Name:
Insulin-like Growth Factor II
Entrez Gene IDs:
3481 (Human); 16002 (Mouse)
Alternate Names:
C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide and methanol, which are known to the State of California to cause cancer and reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression
    Authors: Jang, HJ;Min, HY;Kang, YP;Boo, HJ;Kim, J;Ahn, JH;Oh, SH;Jung, JH;Park, CS;Park, JS;Kim, SY;Lee, HY;
    Nature communications
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. A Gelatin Hydrogel Nonwoven Fabric Combined With Adipose Tissue-Derived Stem Cells Enhances Subcutaneous Islet Engraftment
    Authors: Saito, R;Inagaki, A;Nakamura, Y;Imura, T;Kanai, N;Mitsugashira, H;Endo Kumata, Y;Katano, T;Suzuki, S;Tokodai, K;Kamei, T;Unno, M;Watanabe, K;Tabata, Y;Goto, M;
    Cell transplantation
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Local autocrine plasticity signaling in single dendritic spines by insulin-like growth factors
    Authors: Tu, X;Jain, A;Parra Bueno, P;Decker, H;Liu, X;Yasuda, R;
    Science advances
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for sustainable cultured meat production
    Authors: K Yamanaka, Y Haraguchi, H Takahashi, I Kawashima, T Shimizu
    Scientific Reports, 2023-01-10;13(1):498.
    Species: Bovine
    Sample Types: Cell Culture Supernates.
  5. Comparative Effects of Allulose, Fructose, and Glucose on the Small Intestine
    Authors: T Suzuki, Y Sato, S Kadoya, T Takahashi, M Otomo, H Kobayashi, K Aoki, M Kantake, M Sugiyama, RP Ferraris
    Nutrients, 2022-08-07;14(15):.
    Species: Rat
    Sample Types: Plasma
  6. Critical timing of ACEi initiation prevents compensatory glomerular hypertrophy in the remaining single kidney
    Authors: AS Naik, SQ Wang, M Chowdhury, J Aqeel, CL O'Connor, JE Wiggins, M Bitzer, RC Wiggins
    Scientific Reports, 2021-10-01;11(1):19605.
    Species: Rat
    Sample Types: Urine
  7. Runx2+ Niche Cells Maintain Incisor Mesenchymal Tissue Homeostasis through IGF Signaling
    Authors: S Chen, J Jing, Y Yuan, J Feng, X Han, Q Wen, TV Ho, C Lee, Y Chai
    Cell Rep, 2020-08-11;32(6):108007.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice
    Authors: Y Sui, W Zhang, T Tang, L Gao, T Cao, H Zhu, Q You, B Yu, T Yang
    Stem Cell Res Ther, 2020-02-26;11(1):86.
    Species: Mouse
    Sample Types: Serum

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