Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody
Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody Summary
Ser156-Gln694
Accession # Q80W65
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse and Rat Proprotein Convertase 9/PCSK9 by Western Blot. Western blot shows lysates of mouse liver tissue and rat embryonic cortical neuron. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat Proprotein Convertase 9/PCSK9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3985) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Proprotein Convertase 9/PCSK9 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Proprotein Convertase 9/PCSK9
PC9 (Proprotein convertase subtilisin type 9; also PCSK9 and NARC-1) is a 70‑74 kDa, secreted glycoprotein member of the proteinase K subfamily of subtilases. PC9 functions as a protease that cleaves proproteins at non-basic residues. It also binds to, and promotes, the degradation of LDLR, VLDLR and ApoER2. The mouse PC9 precursor is 694 amino acid (aa) in length, and contains a signal sequence, a 14 kDa, 121 aa prodomain (aa 35‑155), and a 56‑58 kDa, 539 aa mature region (aa 156‑694). The 58 kDa mature region arises from autocatalysis in the ER, and contains a subtilisin-like catalytic domain (aa 166‑432), a modified P-domain (aa 433‑529) and a C-terminal Cys-rich region (aa 530‑694). The prosegment and mature regions remain noncovalently associated. There is one additional PC9 isoform that shows an alternative start site 13 aa upstream of the standard site coupled to a deletion of aa 18‑20. Over aa 156‑694, mouse PC9 shares 93% and 79% aa identity with rat and human PC9, respectively.
Product Datasheets
Citations for Mouse/Rat Proprotein Convertase 9/PCSK9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Rosa26-LSL-dCas9-VPR: a versatile mouse model for tissue specific and simultaneous activation of multiple genes for drug discovery
Authors: D Pakalnišky, T Schönberge, B Strobel, B Stierstorf, T Lamla, M Schuler, M Lenter
Scientific Reports, 2022-11-10;12(1):19268.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
A multienzyme S-nitrosylation cascade regulates cholesterol homeostasis
Authors: CT Stomberski, NM Venetos, HL Zhou, Z Qian, BR Collison, SJ Field, RT Premont, JS Stamler
Cell Reports, 2022-10-25;41(4):111538.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: ICC, Western Blot -
Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice
Authors: EM Haberl, R Pohl, L Rein-Fisch, M Höring, S Krautbauer, G Liebisch, C Buechler
Lipids in Health and Disease, 2021-10-10;20(1):135.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
In vivo adenine base editing of PCSK9 in macaques reduces LDL cholesterol levels
Authors: T Rothgangl, MK Dennis, PJC Lin, R Oka, D Witzigmann, L Villiger, W Qi, M Hruzova, L Kissling, D Lenggenhag, C Borrelli, S Egli, N Frey, N Bakker, JA Walker, AP Kadina, DV Victorov, M Pacesa, S Kreutzer, Z Kontarakis, A Moor, M Jinek, D Weissman, M Stoffel, R van Boxtel, K Holden, N Pardi, B Thöny, J Häberle, YK Tam, SC Semple, G Schwank
Nature Biotechnology, 2021-05-19;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer
Authors: X Liu, X Bao, M Hu, H Chang, M Jiao, J Cheng, L Xie, Q Huang, F Li, CY Li
Nature, 2020-11-11;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Liver Pyruvate Kinase Promotes NAFLD/NASH in both Mice and Humans in a Sex-Specific Manner
Authors: KC Krishnan, RR Floyd, S Sabir, DW Jayasekera, PV Leon-Mimil, AE Jones, AA Cortez, V Shravah, M Péterfy, L Stiles, S Canizales-, AS Divakaruni, A Huertas-Va, AJ Lusis
Cell Mol Gastroenterol Hepatol, 2020-09-14;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis Cas9 in vivo
Authors: R Ibraheim, CQ Song, A Mir, N Amrani, W Xue, EJ Sontheimer
Genome Biol., 2018-09-19;19(1):137.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
?-Secretase Inhibition Lowers Plasma Triglyceride-Rich Lipoproteins by Stabilizing the LDL Receptor
Authors: K Kim, IJ Goldberg, MJ Graham, M Sundaram, E Bertaggia, SX Lee, L Qiang, RA Haeusler, D Metzger, P Chambon, Z Yao, HN Ginsberg, UB Pajvani
Cell Metab., 2018-03-22;0(0):.
Species: Mouse, Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Heparan sulfate proteoglycans present PCSK9 to the LDL receptor
Authors: C Gustafsen, D Olsen, J Vilstrup, S Lund, A Reinhardt, N Wellner, T Larsen, CBF Andersen, K Weyer, JP Li, PH Seeberger, S Thirup, P Madsen, S Glerup
Nat Commun, 2017-09-11;8(1):503.
Species: Hamster, Human
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: Western Blot
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Purified mouse Pcsk9 was used for immunoprecipitation (IP) validation.
5 ug AF3985 per IP incubation, works very well.
This Ab may be further diluted for each IP.
