Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF5418
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Mouse/Rat Transglutaminase 2/TGM2 Biotinylated Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Transglutaminase 2/TGM2 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant human (rh) TGM2 and rhTGM7 is observed, and less than 1% cross‑reactivity with rhTGM3 and rhTGM4 is observed.
Source
Polyclonal Sheep IgG
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Transglutaminase 2/TGM2
Ala2-Ala686
Accession # P21981
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Transglutaminase 2/TGM2 (Catalog # 5418-TG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 2/TGM2

Transglutaminase 2 (TG2), encoded by the Tgm2 gene, is also known as tissue Transglutaminase (tTG), Transglutaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1-3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6-7).

Entrez Gene IDs
7052 (Human); 21817 (Mouse); 56083 (Rat)
Alternate Names
C polypeptide; EC 2.3.2.13; G-ALPHA-h; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; TG(C); TG2; TGase C; TGase H; TGase-2; TGase-H; TGC; TGCGNAH; TGM2; Tissue transglutaminase; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 2; Transglutaminase C; Transglutaminase H; transglutaminase-2; tTG

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