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Mouse/Rat Wnt-5a Antibody

Catalog #: AF645
36 Citations 1 Review Datasheet / COA / SDS
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AF645
AF645-SP
Wnt‑5a in Mouse Embryonic Rib.
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Product Details
Citations (36)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Wnt-5a Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Wnt-5a in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) Wnt‑5b is observed and less than 2% cross-reactivity with rmWnt-1, rmWnt-3a, rmWnt-4, rmWnt-11, and rmWnt-16 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Wnt‑5a peptide
Gln254-Cys334
Accession # P22725
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Lysates of HeLa Human Cervical Epithelial Carcinoma Cells and Mouse Brain (embryo E14)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Wnt‑5a antibody in Mouse Embryonic Rib by Immunohistochemistry (IHC-P). View Larger

Wnt‑5a in Mouse Embryonic Rib. Wnt‑5a was detected in immersion fixed paraffin-embedded sections of mouse embryonic rib using 15 µg/mL Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Wnt-5a antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Wnt‑5a in Mouse Embryo. Wnt-5a was detected in immersion fixed frozen sections of mouse embryo using Mouse/Rat Wnt-5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot View Larger

Detection of Mouse/Rat Wnt‑5a by Western Blot. Western blot shows lysates of HeLa Human Cervical Epithelial Carcinoma Cells and Mouse Brain (embryo E14). PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat Wnt‑5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF645) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Wnt‑5a at approximately ~42kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-5a

Wnt proteins are secreted glycoproteins that contain a conserved pattern of 23-24 cysteine residues. Wnts play critical roles in both carcinogenesis and embryonic development for a variety of organisms. Wnts bind to receptors of the Frizzled family, sometimes in conjunction with other membrane-associated proteins such as LRPs or proteoglycans. Downstream effects of Wnt signaling occur through different intracellular components, depending on which pathway is activated. Three pathways have been characterized: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (1, 2).

Wnt-5a is part of the subgroup of Wnts that are not axis-inducing in Xenopus embryos and do not transform C57MG mammary epithelial cells. This subgroup is also implicated in the Wnt/Ca2+ pathway, playing roles in cell movements and cell adhesion (3). This non-canonical Wnt pathway can inhibit canonical Wnt/ beta -catenin signaling. In Wnt-5a deficient mouse embryos, beta -catenin accumulates in the limb bud suggesting that Wnt-5a normally promotes degradation of beta -catenin (4). Likewise, in Xenopus embryos Wnt-5a antagonizes the ability of the canonical Wnt subgroup to induce a secondary axis (5). Wnt-5a is implicated in various types of cancer and has complex roles. It acts as a tumor suppressor for mammary, B-cell, colon, and uroepithelial cancer cells but is up-regulated in melanomas, where expression levels correlate with severity of metastasis (3). Furthermore, aberrant Wnt-5a signaling results in other diseases such as rheumatoid arthritis (6). Like other developmental growth factors Wnt-5a has diverse roles in development. They are too numerous to enunciate here, as functions span from early anterior-posterior development and gastrulation movements to maintaining hematopoietic stem cell population, lung morphogenesis, and limb outgrowth. Mouse and human Wnt-5a share 97% amino acid identity.

References
  1. Miller, J.R. (2002) Genome Biol. 3:3001.
  2. Roelink, H. and R. Nusse (1991) Genes Dev. 5:381.
  3. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
  4. Topol, L. et al. (2003) J. Cell Biol 162:899.
  5. Torres, M. et al. (1996) J. Cell Biol. 133:1123.
  6. Sen, M. et al. (2001) Arthritis & Rheumatism 44:772.
Long Name
Wingless-type MMTV Integration Site Family, Member 5a
Entrez Gene IDs
7474 (Human); 22418 (Mouse)
Alternate Names
hWNT5A; protein Wnt-5a; wingless-type MMTV integration site family, member 5A; WNT-5A protein; Wnt5a; Wnt-5a

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Citations for Mouse/Rat Wnt-5a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. WNT5A inhibition alters the malignant peripheral nerve sheath tumor microenvironment and enhances tumor growth
    Authors: CS Thomson, J Pundavela, MR Perrino, RA Coover, K Choi, KE Chaney, TA Rizvi, DA Largaespad, N Ratner
    Oncogene, 2021-06-02;40(24):4229-4241.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Coordinated changes in the expression of Wnt pathway genes following human and rat peripheral nerve injury
    Authors: AC van Vliet, J Lee, M van der Po, MRJ Mason, JN Noordermee, LG Fradkin, MR Tannemaat, MJA Malessy, J Verhaagen, F De Winter
    PLoS ONE, 2021-04-13;16(4):e0249748.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Wnt5a expression and prognosis in stage II-III colon cancer
    Authors: CM Lund, A Dyhl-Polk, DL Nielsen, LB Riis
    Transl Oncol, 2020-10-09;14(1):100892.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency
    Authors: W Kawarazaki, R Mizuno, M Nishimoto, N Ayuzawa, D Hirohama, K Ueda, F Kawakami-M, S Oba, T Marumo, T Fujita
    J. Clin. Invest., 2020-08-03;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  5. Inhibition of colony stimulating factor 1 receptor corrects maternal inflammation-induced microglial and synaptic dysfunction and behavioral abnormalities
    Authors: S Ikezu, H Yeh, JC Delpech, ME Woodbury, AA Van Enoo, Z Ruan, S Sivakumara, Y You, C Holland, T Guillamon-, A Yoshii-Kit, MB Botros, C Madore, PH Chao, A Desani, S Manimaran, SV Kalavai, WE Johnson, O Butovsky, M Medalla, JI Luebke, T Ikezu
    Mol. Psychiatry, 2020-02-18;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Detection
  6. Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors
    Authors: X Wu, EM van Dijk, JP Ng-Blichfe, IST Bos, C Ciminieri, M Königshoff, LEM Kistemaker, R Gosens
    Cells, 2019-09-25;8(10):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Wnt5a Contributes to the Differentiation of Human Embryonic Stem Cells into Lentoid Bodies Through the Noncanonical Wnt/JNK Signaling Pathway
    Authors: C Han, J Li, C Wang, H Ouyang, X Ding, Y Liu, S Chen, L Luo
    Invest. Ophthalmol. Vis. Sci., 2018-07-02;59(8):3449-3460.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Reduced Cdc42 Activity Compromises Hematopoiesis-Supportive Function Of Fanconi Anemia Mesenchymal Stromal Cells
    Authors: J Xu, X Li, A Cole, Z Sherman, W Du
    Stem Cells, 2018-02-09;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. ERAD-dependent control of the Wnt secretory factor Evi
    Authors: K Glaeser, M Urban, E Fenech, O Voloshanen, D Kranz, F Lari, JC Christians, M Boutros
    EMBO J., 2018-01-29;37(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Wnt5a is essential for hippocampal dendritic maintenance and spatial learning and memory in adult mice
    Authors: CM Chen, LL Orefice, SL Chiu, TA LeGates, S Hattar, RL Huganir, H Zhao, B Xu, R Kuruvilla
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-09;114(4):E619-E628.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. Noncanonical WNT-5A signaling impairs endogenous lung repair in COPD
    Authors: Hoeke A Baarsma
    J. Exp. Med, 2016-12-15;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development
    Sci Rep, 2016-08-26;6(0):32094.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  13. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016-07-11;0(0):.
    Applications: Bioassay
  14. IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling
    PLoS ONE, 2016-05-23;11(5):e0156002.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  15. Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells
    Authors: T Skaria, E Bachli, G Schoedon
    Cell Adh Migr, 2016-05-09;11(1):24-38.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
    Authors: Bruce F Kimler
    J. Endocrinol., 2016-03-14;229(2):197-207.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  17. Thyroid hormone-regulated Wnt5a/Ror2 signaling is essential for dedifferentiation of larval epithelial cells into adult stem cells in the Xenopus laevis intestine.
    Authors: Ishizuya-Oka, Atsuko, Kajita, Mitsuko, Hasebe, Takashi
    PLoS ONE, 2014-09-11;9(9):e107611.
    Species: Xenopus
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Blocking, Western Blot
  18. WNT5A induces release of exosomes containing pro-angiogenic and immunosuppressive factors from malignant melanoma cells.
    Authors: Ekstrom E, Bergenfelz C, von Bulow V, Serifler F, Carlemalm E, Jonsson G, Andersson T, Leandersson K
    Mol Cancer, 2014-04-26;13(0):88.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. TGF-beta-activated kinase 1 (TAK1) signaling regulates TGF-beta-induced WNT-5A expression in airway smooth muscle cells via Sp1 and beta-catenin.
    Authors: Kumawat K, Menzen M, Slegtenhorst R, Halayko A, Schmidt M, Gosens R
    PLoS ONE, 2014-04-11;9(4):e94801.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.
    Authors: Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J
    Dev Biol, 2013-11-08;385(2):291-303.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing.
    Authors: Florian M, Nattamai K, Dorr K, Marka G, Uberle B, Vas V, Eckl C, Andra I, Schiemann M, Oostendorp R, Scharffetter-Kochanek K, Kestler H, Zheng Y, Geiger H
    Nature, 2013-10-20;503(7476):392-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  22. Ror family receptor tyrosine kinases regulate the maintenance of neural progenitor cells in the developing neocortex.
    J. Cell. Sci., 2012-02-10;125(0):2017-29.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. NMDA receptor activation stimulates transcription-independent rapid wnt5a protein synthesis via the MAPK signaling pathway.
    Mol Brain, 2012-01-04;5(0):1.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  24. WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity.
    Authors: Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ
    PLoS ONE, 2011-08-17;6(8):e22920.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  25. Wnt5a suppresses epithelial ovarian cancer by promoting cellular senescence.
    Authors: Bitler BG, Nicodemus JP, Li H, Cai Q, Wu H, Hua X, Li T, Birrer MJ, Godwin AK, Cairns P, Zhang R
    Cancer Res., 2011-08-04;71(19):6184-94.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  26. Evidence for altered Wnt signaling in psoriatic skin.
    Authors: Gudjonsson JE, Johnston A, Stoll SW
    J. Invest. Dermatol., 2010-04-08;130(7):1849-59.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Bioassay, IHC-P
  27. Wnt5a mediates nerve growth factor-dependent axonal branching and growth in developing sympathetic neurons.
    Authors: Bodmer D, Levine-Wilkinson S, Richmond A, Hirsh S, Kuruvilla R
    J. Neurosci., 2009-06-10;29(23):7569-81.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  28. Wnt5a is expressed in murine and human atherosclerotic lesions.
    Authors: Christman MA, Goetz DJ, Dickerson E, McCall KD, Lewis CJ, Benencia F, Silver MJ, Kohn LD, Malgor R
    Am. J. Physiol. Heart Circ. Physiol., 2008-05-02;294(6):H2864-70.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. Wnt5A/CaMKII signaling contributes to the inflammatory response of macrophages and is a target for the antiinflammatory action of activated protein C and interleukin-10.
    Authors: Pereira C, Schaer DJ, Bachli EB, Kurrer MO, Schoedon G
    Arterioscler. Thromb. Vasc. Biol., 2008-01-03;28(3):504-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  30. Dickkopf-1 is a master regulator of joint remodeling.
    Authors: Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
    Nat. Med., 2007-01-21;13(2):156-63.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  31. Wnt5a signaling induces proliferation and survival of endothelial cells in vitro and expression of MMP-1 and Tie-2.
    Authors: Masckauchan TN, Agalliu D, Vorontchikhina M, Ahn A, Parmalee NL, Li CM, Khoo A, Tycko B, Brown AM, Kitajewski J
    Mol. Biol. Cell, 2006-10-11;17(12):5163-72.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. The Wingless homolog WNT5A and its receptor Frizzled-5 regulate inflammatory responses of human mononuclear cells induced by microbial stimulation.
    Authors: Blumenthal A, Ehlers S, Lauber J, Buer J, Lange C, Goldmann T, Heine H, Brandt E, Reiling N
    Blood, 2006-04-06;108(3):965-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  33. Ventral midbrain glia express region-specific transcription factors and regulate dopaminergic neurogenesis through Wnt-5a secretion.
    Authors: Castelo-Branco G, Sousa KM, Bryja V, Pinto L, Wagner J, Arenas E
    Mol. Cell. Neurosci., 2005-10-21;31(2):251-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  34. Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.
    Authors: Polesskaya A, Seale P, Rudnicki MA
    Cell, 2003-06-27;113(7):841-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Wnt-5A augments repopulating capacity and primitive hematopoietic development of human blood stem cells in vivo.
    Authors: Murdoch B, Chadwick K, Martin M, Shojaei F, Shah KV, Gallacher L, Moon RT, Bhatia M
    Proc. Natl. Acad. Sci. U.S.A., 2003-03-07;100(6):3422-7.
    Species: Xenopus
    Sample Types: Cell Culture Supernates
    Applications: Immunodepletion
  36. Signaling of rat Frizzled-2 through phosphodiesterase and cyclic GMP.
    Authors: Ahumada A, Slusarski DC, Liu X, Moon RT, Malbon CC, Wang HY
    Science, 2002-12-06;298(5600):2006-10.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunodepletion

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Mouse/Rat Wnt-5a Antibody
By Anonymous on 02/19/2018
Application: WB Sample Tested: B16-F1 mouse melanoma cell line Species: Mouse

Non-specific bands at 64kDa, actual band at 49KDa. Recommend to use 7.5% gel for better separation.

Western Blot Mouse/Rat Wnt-5a Antibody AF645