Mouse RBP4/Retinol-Binding Protein 4 Antibody

Catalog #: AF3476 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3476
AF3476-SP

Detection of Mouse RBP4/Retinol‑Binding Protein 4 by Western Blot.

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All RBP4/Retinol-Binding Protein 4 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse RBP4/Retinol-Binding Protein 4 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse RBP4 in direct ELISAs and Western blots. In these formats, approximately 10% cross‑reactivity with recombinant human RBP4 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RBP4 (R&D Systems, Catalog # 3476-LC)
Glu19-Leu201
Accession # Q00724

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse RBP4/Retinol‑Binding Protein 4 by Western Blot. Western blot shows lysate of Hepa 1-6 mouse hepatoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Mouse RBP4/Retinol-Binding Protein 4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3476) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RBP4/Retinol-Binding Protein 4 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution. However, retinol becomes quite stable and soluble in plasma due to its tight interaction with retinol-binding protein 4 (RBP4), also known as plasma retinol-binding protein (1 - 3). A prototypic member of the lipocalin superfamily, RBP4 has a beta ‑barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision. Serum RBP4 levels are elevated in insulin-resistant mice and humans with obesity and type 2 diabetes, implying that RBP4, an adipocyte-derived signal, may be a biomarker and a drug target for the two diseases.

References