Mouse Resistin Biotinylated Antibody

Catalog #: BAF1069
2 Citations Datasheet / COA / SDS

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BAF1069

All Resistin Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Resistin Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Resistin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) Resistin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Resistin (R&D Systems, Catalog # 1069-RN)
Ser21-Ser114
Accession # Q99P87

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Resistin (Catalog # 1069-RN)

Mouse Resistin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse Resistin Antibody (Catalog # MAB1069)

Standard: Recombinant Mouse Resistin Protein (Catalog # 1069-RN)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Resistin

Resistin, also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a member of a family of secreted cysteine-rich peptide hormones that also includes Resistin-like molecules RELM alpha, beta, and gamma. These molecules play important roles in inflammation, glucose metabolism, and insulin resistance (1, 2). Mature mouse Resistin is a 12 kDa protein with an N-terminal alpha -helical domain and a C-terminal beta -sandwich domain that is stabilized by multiple intrachain disulfide bonds (3). Resistin circulates as noncovalent trimers and disulfide-linked hexamers, with the trimeric form showing greater bioactivity (3, 4). Resistin can also form multimers with RELM beta (4). Mature mouse Resistin shares 56% and 72% amino acid (aa) sequence identity with human and rat Resistin, respectively. It shares 34% - 42% aa sequence identity with mouse RELM alpha, beta, and gamma. In rodents, Resistin is expressed by adipocytes and in the pituitary and arcuate nucleus of the hypothalamus (5 - 9). It is upregulated during adipogenesis, in obesity, and by insulin or a high carbohydrate diet (5, 6). This is in contrast to human Resistin which is produced by macrophages and monocytes but not by adipocytes (9 - 12). Mouse Resistin induces proinflammatory molecule production in adipocytes and promotes hepatic gluconeogenesis and insulin resistance (5, 7). Human Resistin promotes lipolysis by human and mouse adipocytes, but mouse Resistin does not promote lipolysis by adipocytes of either species (10). Both mouse and human Resistin promote vascular endothelial cell sprouting in vitro and inflammatory reactions in vivo (13 - 16).

References

Adeghate, E. (2004) Cell. Mol. Life Sci. 61:2485.
Asano, T. et al. (2006) Curr. Diabetes Rev. 2:449.
Patel, S.D. et al. (2004) Science 304:1154.
Chen, J. et al. (2002) J. Endocrinol. 175:499.
Steppan, C.M. et al. (2001) Nature 409:307.
Kim, K-H. et al. (2001) J. Biol. Chem. 276:11252.
Banerjee, R.R. et al. (2004) Science 303:1195.
Rajala, M.W. et al. (2002) Mol. Endocrinol. 16:1920.
Morash, B.A. et al. (2002) FEBS Lett. 526:26.
Ort, T. et al. (2005) Endocrinology 146:2200.
Nagaev, I. et al. (2006) Plos ONE 1:e31.
Savage, D.B. et al. (2001) Diabetes 50:2199.
Mu, H. et al. (2006) Cardiovasc. Res. 70:146.
Robertson, S.A. et al. (2008) Regul. Pept. August 5 epub.
Bokarewa, M. et al. (2005) J. Immunol. 174:5789.
Burnett, M.S. et al. (2005) Atherosclerosis 182:241.

Entrez Gene IDs

56729 (Human); 57264 (Mouse)

Alternate Names

Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1

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Citations for Mouse Resistin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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