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Mouse S100A8 Antibody

Catalog #: MAB3059
12 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3059
MAB3059-SP
Detection of S100A8 Rat by Western Blot.
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Product Details
Citations (12)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse S100A8 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 40% cross-reactivity with recombinant human (rh) S100A8 is observed and no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A9, rmS100A10, rmS100A11, rhS100B, or rhS100P is observed.
Source
Monoclonal Rat IgG2B Clone # 335806
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse S100A8
Met1-Glu89 (predicted)
Accession # P27005
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of S100A8 Rat antibody by Western Blot. View Larger

Detection of S100A8 Rat by Western Blot. Western blot shows lysates of mouse leukocyte. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry S100A8 antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

S100A8 in NIH-3T3 Mouse Cell Line. S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10 µg/mL Rat Anti-Mouse S100A8 Monoclonal Antibody (Catalog # MAB3059) for 3 hours at room temperature. Cells were stained (red) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A8

Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10 kDa member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal alpha -helix, followed by a calcium-binding EF-hand segment, and a C-terminal alpha -helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 shares 80% and 57% aa sequence identity with rat and human S100A8, respectively.

Long Name
S100 Calcium Binding Protein A8
Entrez Gene IDs
6279 (Human); 20201 (Mouse)
Alternate Names
60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A

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Citations for Mouse S100A8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Crisaborole Inhibits Itch and Pain by Preventing Neutrophil Infiltration in a Mouse Model of Atopic Dermatitis
    Authors: Darya Pavlenko, Zeynep Todurga Todurga Seven, Lauren Bystrom, Anika Markan, Rebecca Verpile, Hirotake Ishida et al.
    Acta Dermato-Venereologica
  2. Alterations in Wnt- and/or STAT3 signaling pathways and the immune microenvironment during metastatic progression
    Authors: S. J. Kim, S. Garcia-Recio, C. J. Creighton, C. M. Perou, J. M. Rosen
    Oncogene
  3. S100a9 Protects Male Lupus-Prone NZBWF1 Mice From Disease Development
    Authors: Laura M. Davison, Andres A. Alberto, Hardik A. Dand, Emma J. Keller, Madeline Patt, Ayesha Khan et al.
    Frontiers in Immunology
  4. Single-Cell Analysis Unveils the Role of the Tumor Immune Microenvironment and Notch Signaling in Dormant Minimal Residual Disease
    Authors: Mahnaz Janghorban, Yuchen Yang, Na Zhao, Clark Hamor, Tuan M. Nguyen, Xiang H.-F. Zhang et al.
    Cancer Research
  5. Targeting EIF4A triggers an interferon response to synergize with chemotherapy and suppress triple-negative breast cancer
    Authors: Zhao, N;Kabotyanski, EB;Saltzman, AB;Malovannaya, A;Yuan, X;Reineke, LC;Lieu, N;Gao, Y;Pedroza, DA;Calderon, SJ;Smith, AJ;Hamor, C;Safari, K;Savage, S;Zhang, B;Zhou, J;Solis, LM;Hilsenbeck, SG;Fan, C;Perou, CM;Rosen, JM;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Single-cell evaluation reveals shifts in the tumor-immune niches that shape and maintain aggressive lesions in the breast
    Authors: VC Sinha, AL Rinkenbaug, M Xu, X Zhou, X Zhang, S Jeter-Jone, J Shao, Y Qi, JA Zebala, DY Maeda, F McAllister, H Piwnica-Wo
    Nature Communications, 2021-08-18;12(1):5024.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development
    Authors: N Makthal, K Nguyen, H Do, M Gavagan, P Chandrangs, JD Helmann, RJ Olsen, M Kumaraswam
    EBioMedicine, 2017-06-02;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Visualized macrophage dynamics and significance of S100A8 in obese fat.
    Authors: Sekimoto R, Fukuda S, Maeda N, Tsushima Y, Matsuda K, Mori T, Nakatsuji H, Nishizawa H, Kishida K, Kikuta J, Maijima Y, Funahashi T, Ishii M, Shimomura I
    Proc Natl Acad Sci U S A, 2015-04-06;112(16):E2058-66.
    Species: Mouse
    Sample Types: Whole Tissue
  9. The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion.
    Authors: Dessing, Mark C, Tammaro, Alessand, Pulskens, Wilco P, Teske, Gwendoli, Butter, Loes M, Claessen, Nike, van Eijk, Marco, van der Poll, Tom, Vogl, Thomas, Roth, Johannes, Florquin, Sandrine, Leemans, Jaklien
    Kidney Int, 2014-06-18;87(1):85-94.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway.
    Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J
    PLoS ONE, 2012-09-25;7(9):e46311.
    Species: Mouse
    Sample Types: Vaginal Lavage Fluid, Whole Tissue
    Applications: ELISA Development, IHC-Fr
  11. S100A8/A9 is not involved in host defense against murine urinary tract infection.
    Authors: Dessing MC, Butter LM, Teske GJ
    PLoS ONE, 2010-10-14;5(10):e13394.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Loss of H2R signaling disrupts neutrophil homeostasis and promotes inflammation-associated colonic tumorigenesis in mice
    Authors: Z Shi, Y Mori-Akiya, W Du, R Fultz, Y Zhao, W Ruan, S Venable, MA Engevik, J Versalovic
    Cellular and Molecular Gastroenterology and Hepatology, 2021-11-13;0(0):.

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Mouse S100A8 Antibody
By Anonymous on 11/02/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Artery Smooth Muscle Cells,Pulmonary Artery Smooth Muscle Cells Species: Mouse